Structural relationships among the H-2 D-regions of murine MHC haplotypes

L. W. Duran, R. M. Horton, C. W. Birschbach, A. Chang-miller, L. R. Pease

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19 Scopus citations

Abstract

The number of genes encoding functional Ag-presenting molecules in the D region of the murine MHC differs among haplotypes. For example, the H-2b D region contains a single 'D/L' gene, H-2Db, whereas the d-haplotype encodes two, H-2D(d) and L(d). Using D/L specific oligonucleotide probes, we have found that, as with H-2(d), the q- and v-haplotypes contain two D/L genes, whereas the other haplotypes examined have one. Hybridization analysis using cloned probes that map between H-2D(d) and L(d) revealed similar structures in each of the three haplotypes (d, q, and v) which have 'duplicated' D regions. Two approaches were used to examine allelic relationships among the D/L genes. First, the 5' region of the H-2Db was sequenced, and found to be more similar to H-2L(d) than to H-2D(d). Second, oligonucleotide probes that distinguish H-2L(d) from H-2D(d) revealed H-2L(d)-related genes in several haplotypes, including the duplicated haplotypes H-2(q) and H-2(v). Analogous probes specific for H-2D(d), however, did not detect similar sequences in the other haplotypes. We interpret these results to mean that the three duplicated D regions arose from a common duplication event, and share the five gene structure of the D region cluster defined in H-2(d). However, subsequent events have generated sequence divergence at the D-locus.

Original languageEnglish (US)
Pages (from-to)288-296
Number of pages9
JournalJournal of Immunology
Volume142
Issue number1
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Duran, L. W., Horton, R. M., Birschbach, C. W., Chang-miller, A., & Pease, L. R. (1989). Structural relationships among the H-2 D-regions of murine MHC haplotypes. Journal of Immunology, 142(1), 288-296.