Structural properties of the adipocyte lipid binding protein

Amy Reese-Wagoner, James Thompson, Leonard Banaszak

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The adipocyte lipid binding protein, ALBP (also adipocyte fatty acid binding protein, A-FABP, 422 protein, aP2, and p15 protein), is one of the most studied of the intracellular lipid binding protein family. Here we sequentially compare the different sources of ALBP and describe the idea that one-third of the amino acid side chains near the N-terminal end appear to play a major role in conformational dynamics and in ligand transfer. Crystallographic data for mouse ALBP are summarized and the ligand binding cavity analyzed in terms of the overall surface and conformational dynamics. The region of the proposed ligand portal is described. Amino acid side chains critical to cavity formation and fatty acid interactions are analyzed by comparing known crystal structures containing a series of different hydrophobic ligands. Finally, we address ALBP ligand binding affinity and thermodynamic studies. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)106-116
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1441
Issue number2-3
DOIs
StatePublished - Nov 23 1999
Externally publishedYes

Fingerprint

Fatty Acid-Binding Proteins
Ligands
Amino Acids
Staphylococcal Protein A
Thermodynamics
Adipocytes
Carrier Proteins
Proteins
Fatty Acids
Lipids

Keywords

  • β-Barrel protein
  • Adipocyte
  • Fatty acid binding protein
  • Intracellular lipid binding protein

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Structural properties of the adipocyte lipid binding protein. / Reese-Wagoner, Amy; Thompson, James; Banaszak, Leonard.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1441, No. 2-3, 23.11.1999, p. 106-116.

Research output: Contribution to journalArticle

Reese-Wagoner, Amy ; Thompson, James ; Banaszak, Leonard. / Structural properties of the adipocyte lipid binding protein. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 1999 ; Vol. 1441, No. 2-3. pp. 106-116.
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