Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules

I. J. Griffith, N. Nabavi, Z. Ghogawala, C. G. Chase, M. Rodriguez, D. J. McKean, L. H. Glimcher

Research output: Contribution to journalArticle

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Abstract

We have selected Ia variants from the Ia+ (H-2(d) M12.4.1 B cell lymphoma that are negative on the cell surface for one or both Ia isotypes. The molecular analysis of two such independently selected cell lines, M12.A2 and M12.C3, is reported here. This analysis revealed that the genes encoding A(β)(d) (M12.A2) and E(β)(d) (M12.C3) contained identical single-nucleotide transitions that resulted in the substitution of Ser (mutant) for Asn (wild-type) at residue 82/83 of the extracellular NH2-terminal (membrane distal) β1 domain. This conservative substitution caused a cytoplasmic accumulation of I-A or I-E molecules in the respective cell line although predicted secondary-structure analysis suggests a minimal effect on protein conformation. Thus, the mutation appears to have either created a negative signal that stops transport or eliminated a positive signal that is required for transport and targeting to the cell surface.

Original languageEnglish (US)
Pages (from-to)541-555
Number of pages15
JournalJournal of Experimental Medicine
Volume167
Issue number2
StatePublished - 1988
Externally publishedYes

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varespladib methyl
Cell Line
Protein Conformation
Mutation
B-Cell Lymphoma
Nucleotides
Membranes
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Griffith, I. J., Nabavi, N., Ghogawala, Z., Chase, C. G., Rodriguez, M., McKean, D. J., & Glimcher, L. H. (1988). Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules. Journal of Experimental Medicine, 167(2), 541-555.

Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules. / Griffith, I. J.; Nabavi, N.; Ghogawala, Z.; Chase, C. G.; Rodriguez, M.; McKean, D. J.; Glimcher, L. H.

In: Journal of Experimental Medicine, Vol. 167, No. 2, 1988, p. 541-555.

Research output: Contribution to journalArticle

Griffith, IJ, Nabavi, N, Ghogawala, Z, Chase, CG, Rodriguez, M, McKean, DJ & Glimcher, LH 1988, 'Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules', Journal of Experimental Medicine, vol. 167, no. 2, pp. 541-555.
Griffith IJ, Nabavi N, Ghogawala Z, Chase CG, Rodriguez M, McKean DJ et al. Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules. Journal of Experimental Medicine. 1988;167(2):541-555.
Griffith, I. J. ; Nabavi, N. ; Ghogawala, Z. ; Chase, C. G. ; Rodriguez, M. ; McKean, D. J. ; Glimcher, L. H. / Structural mutation affecting intracellular transport and cell surface expression of murine class II molecules. In: Journal of Experimental Medicine. 1988 ; Vol. 167, No. 2. pp. 541-555.
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