Structural Insights into the Extracytoplasmic Thiamine-Binding Lipoprotein p37 of Mycoplasma hyorhinis

Katherine H. Sippel, Arthur H. Robbins, Robbie Reutzel, Susan K. Boehlein, Kazunori Namiki, Steven Goodison, Mavis Agbandje-McKenna, Charles J. Rosser, Robert McKenna

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The Mycoplasma hyorhinis protein p37 has been implicated in tumorigenic transformation for more than 20 years. Though there are many speculations as to its function, based solely on sequence homology, the issue has remained unresolved. Presented here is the 1.6-A°-resolution refined crystal structure of M. hyorhinis p37, renamed the extracytoplasmic thiamine-binding lipoprotein (Cypl). The structure shows thiamine pyrophosphate (TPP) and two calcium ions are bound to Cypl and give the first insights into possible functions of the Cypl-like family of proteins. Sequence alignments of Cypl-like proteins between several different species of mycoplasma show that the thiamine-binding site is likely conserved and structural alignments reveal the similarity of Cypl to various binding proteins. While the experimentally determined function of Cypl remains unknown, the structure shows that the protein is a TPP-binding protein, opening up many avenues for future mechanistic studies and making Cypl a possible target for combating mycoplasma infections and tumorigenic transformation.

Original languageEnglish (US)
Pages (from-to)2585-2592
Number of pages8
JournalJournal of Bacteriology
Volume191
Issue number8
DOIs
StatePublished - Apr 1 2009
Externally publishedYes

Fingerprint

Mycoplasma hyorhinis
Thiamine
Lipoproteins
Neoplastic Cell Transformation
Thiamine Pyrophosphate
Proteins
Mycoplasma Infections
Mycoplasma
Sequence Alignment
Sequence Homology
Carrier Proteins
Binding Sites
Ions
Calcium

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Sippel, K. H., Robbins, A. H., Reutzel, R., Boehlein, S. K., Namiki, K., Goodison, S., ... McKenna, R. (2009). Structural Insights into the Extracytoplasmic Thiamine-Binding Lipoprotein p37 of Mycoplasma hyorhinis. Journal of Bacteriology, 191(8), 2585-2592. https://doi.org/10.1128/JB.01680-08

Structural Insights into the Extracytoplasmic Thiamine-Binding Lipoprotein p37 of Mycoplasma hyorhinis. / Sippel, Katherine H.; Robbins, Arthur H.; Reutzel, Robbie; Boehlein, Susan K.; Namiki, Kazunori; Goodison, Steven; Agbandje-McKenna, Mavis; Rosser, Charles J.; McKenna, Robert.

In: Journal of Bacteriology, Vol. 191, No. 8, 01.04.2009, p. 2585-2592.

Research output: Contribution to journalArticle

Sippel, KH, Robbins, AH, Reutzel, R, Boehlein, SK, Namiki, K, Goodison, S, Agbandje-McKenna, M, Rosser, CJ & McKenna, R 2009, 'Structural Insights into the Extracytoplasmic Thiamine-Binding Lipoprotein p37 of Mycoplasma hyorhinis', Journal of Bacteriology, vol. 191, no. 8, pp. 2585-2592. https://doi.org/10.1128/JB.01680-08
Sippel, Katherine H. ; Robbins, Arthur H. ; Reutzel, Robbie ; Boehlein, Susan K. ; Namiki, Kazunori ; Goodison, Steven ; Agbandje-McKenna, Mavis ; Rosser, Charles J. ; McKenna, Robert. / Structural Insights into the Extracytoplasmic Thiamine-Binding Lipoprotein p37 of Mycoplasma hyorhinis. In: Journal of Bacteriology. 2009 ; Vol. 191, No. 8. pp. 2585-2592.
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