Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA

Georges Mer, Alexey Bochkarev, Rajesh Gupta, Elena Bochkareva, Lori Frappier, C. James Ingles, Aled M. Edwards, Walter J. Chazin

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Replication protein A (RPA), the nuclear ssDNA-binding protein in eukaryotes, is essential to DNA replication, recombination, and repair. We have shown that a globular domain at the C terminus of subunit RPA32 contains a specific surface that interacts in a similar manner with the DNA repair enzyme UNG2 and repair factors XPA and RAD52, each of which functions in a different repair pathway. NMR structures of the RPA32 domain, free and in complex with the minimal interaction domain of UNG2, were determined, defining a common structural basis for linking RPA to the nucleotide excision, base excision, and recombinational pathways of repairing damaged DNA. Our findings support a hand-off model for the assembly and coordination of different components of the DNA repair machinery.

Original languageEnglish (US)
Pages (from-to)449-456
Number of pages8
JournalCell
Volume103
Issue number3
StatePublished - Oct 27 2000
Externally publishedYes

Fingerprint

Replication Protein A
DNA Repair
Repair
DNA Repair Enzymes
Recombinational DNA Repair
DNA
Eukaryota
DNA Replication
Carrier Proteins
Proteins
Nucleotides
Hand
Machinery
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Mer, G., Bochkarev, A., Gupta, R., Bochkareva, E., Frappier, L., Ingles, C. J., ... Chazin, W. J. (2000). Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. Cell, 103(3), 449-456.

Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. / Mer, Georges; Bochkarev, Alexey; Gupta, Rajesh; Bochkareva, Elena; Frappier, Lori; Ingles, C. James; Edwards, Aled M.; Chazin, Walter J.

In: Cell, Vol. 103, No. 3, 27.10.2000, p. 449-456.

Research output: Contribution to journalArticle

Mer, G, Bochkarev, A, Gupta, R, Bochkareva, E, Frappier, L, Ingles, CJ, Edwards, AM & Chazin, WJ 2000, 'Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA', Cell, vol. 103, no. 3, pp. 449-456.
Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles CJ et al. Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. Cell. 2000 Oct 27;103(3):449-456.
Mer, Georges ; Bochkarev, Alexey ; Gupta, Rajesh ; Bochkareva, Elena ; Frappier, Lori ; Ingles, C. James ; Edwards, Aled M. ; Chazin, Walter J. / Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. In: Cell. 2000 ; Vol. 103, No. 3. pp. 449-456.
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