Structural basis for α-bungarotoxin insensitivity of neuronal nicotinic acetylcholine receptors

Steven M Sine, John R. Strikwerda, Simone Mazzaferro

Research output: Contribution to journalArticle

Abstract

The ten types of nicotinic acetylcholine receptor α-subunits show substantial sequence homology, yet some types confer high affinity for α-bungarotoxin, whereas others confer negligible affinity. Combining sequence alignments with structural data reveals three residues unique to α-toxin-refractory α-subunits that coalesce within the 3D structure of the α4β2 receptor and are predicted to fit between loops I and II of α-bungarotoxin. Mutating any one of these residues, Lys189, Ile196 or Lys153, to the α-toxin-permissive counterpart fails to confer α-bungarotoxin binding. However, mutating both Lys189 and Ile196 affords α-bungarotoxin binding with an apparent dissociation constant of 104 nM, while combining mutation of Lys153 reduces the dissociation constant to 22 nM. Analogous residue substitutions also confer high affinity α-bungarotoxin binding upon α-toxin-refractory α2 and α3 subunits. α4β2 receptors engineered to bind α-bungarotoxin exhibit slow rates of α-toxin association and dissociation, and competition by cholinergic ligands typical of muscle nicotinic receptors. Receptors engineered to bind α-bungarotoxin co-sediment with muscle nicotinic receptors on sucrose gradients, and mirror single channel signatures of their α-toxin-refractory counterparts. Thus the inability of α-bungarotoxin to bind to neuronal nicotinic receptors arises from three unique and interdependent residues that coalesce within the receptor's 3D structure.

Original languageEnglish (US)
Article number107660
JournalNeuropharmacology
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Bungarotoxins
Nicotinic Receptors
Muscles
neuronal bungarotoxin
Sequence Alignment
Sequence Homology
Cholinergic Agents
Sucrose
Ligands
Mutation

Keywords

  • 3D structure
  • Inter-residue interactions
  • Neuronal nicotinic acetylcholine receptor
  • Radio-ligand binding
  • Single channel recording
  • Sucrose gradient analysis
  • α-Bungarotoxin
  • α-subunit

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

Cite this

Structural basis for α-bungarotoxin insensitivity of neuronal nicotinic acetylcholine receptors. / Sine, Steven M; Strikwerda, John R.; Mazzaferro, Simone.

In: Neuropharmacology, 01.01.2019.

Research output: Contribution to journalArticle

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