Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells

C. R. Shea, D. Whitaker, G. F. Murphy, N. Chen, J. Wimberly, M. Scholz, M. E. Sherwood, Thomas J Flotte, T. Hasan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In order to elucidate the mechanisms of photosensitized injury to mitochondria, two photosensitizers have been compared. Both doxycycline (DOTC) and rhodamine-123 (R123) localize preferentially within the mitochondria of MGH-U1 bladder carcinoma cells in vitro, and both sensitize phototoxic injury that is selective for mitochondria. Mitochondria of cells pretreated with DOTC and irradiated with UVA (1 J/cm2, 320-400 nm) undergo massive swelling that begins by 10 min after irradiation, is maximal by 1 h, and is partially repaired by 4 h; damage caused by exposure to a higher UVA dose (6 J/cm2), however, is not repaired. In contrast, cells pretreated with R123 and irradiated with an argon-ion laser (10 J/cm2, 514.5 nm) undergo a different type of mitochondrial injury, characterized by the delayed (4 h) onset of moderate mitochondrial swelling and striking mitochondrial distortion and fragmentation, which is not repaired by 48 h after irradiation. These differences indicated that the reactions underlying cellular photoxicity can be distinguished even on an ultrastructural level. Probably both the primary photochemistry and the submitochondrial targets of these reactions differ with the two photosensitizers.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsSteven L. Jacques
Place of PublicationBellingham, WA, United States
PublisherPubl by Int Soc for Optical Engineering
Pages80-87
Number of pages8
Volume1202
ISBN (Print)0819402435
StatePublished - 1990
Externally publishedYes
EventProceedings of Laser-Tissue Interaction - Los Angeles, CA, USA
Duration: Jan 15 1990Jan 17 1990

Other

OtherProceedings of Laser-Tissue Interaction
CityLos Angeles, CA, USA
Period1/15/901/17/90

Fingerprint

Mitochondria
mitochondria
rhodamine
Photosensitizers
cells
swelling
Swelling
Irradiation
irradiation
bladder
Photochemical reactions
photochemical reactions
Argon
fragmentation
cancer
Cells
argon
damage
dosage
Lasers

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Shea, C. R., Whitaker, D., Murphy, G. F., Chen, N., Wimberly, J., Scholz, M., ... Hasan, T. (1990). Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells. In S. L. Jacques (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1202, pp. 80-87). Bellingham, WA, United States: Publ by Int Soc for Optical Engineering.

Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells. / Shea, C. R.; Whitaker, D.; Murphy, G. F.; Chen, N.; Wimberly, J.; Scholz, M.; Sherwood, M. E.; Flotte, Thomas J; Hasan, T.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Steven L. Jacques. Vol. 1202 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1990. p. 80-87.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shea, CR, Whitaker, D, Murphy, GF, Chen, N, Wimberly, J, Scholz, M, Sherwood, ME, Flotte, TJ & Hasan, T 1990, Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells. in SL Jacques (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1202, Publ by Int Soc for Optical Engineering, Bellingham, WA, United States, pp. 80-87, Proceedings of Laser-Tissue Interaction, Los Angeles, CA, USA, 1/15/90.
Shea CR, Whitaker D, Murphy GF, Chen N, Wimberly J, Scholz M et al. Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells. In Jacques SL, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1202. Bellingham, WA, United States: Publ by Int Soc for Optical Engineering. 1990. p. 80-87
Shea, C. R. ; Whitaker, D. ; Murphy, G. F. ; Chen, N. ; Wimberly, J. ; Scholz, M. ; Sherwood, M. E. ; Flotte, Thomas J ; Hasan, T. / Structural and functional alterations in rhodamine-123- and doxycycline-photosensitized cells. Proceedings of SPIE - The International Society for Optical Engineering. editor / Steven L. Jacques. Vol. 1202 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1990. pp. 80-87
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