Structural analysis of H-2K(f) and H-2K(f)m1 by using H-2K locus-specific sequences

R. M. Horton, W. H. Hildebrand, J. M. Martinko, L. R. Pease

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The H-2K(f) allele and the spontaneous mutant K(f)m1 have been cloned using locus-specific sequences. The mutation consists of a cluster of four nucleotide changes, resulting in amino acid substitutions at positions 95 (Leu→Ile) and 97 (Val→Arg). This finding has structural, genetic, and technical implications. The amino acid substitutions are located on the β-strands of the antigen recognition site. Their influence on the allogeneic properties of the K(f) glycoprotein is consistent with the hypothesis that alloreactivity results from alterations in the spectrum of peptides presented to T cells. These substitutions would not, however, be predicted to be directly accessable for binding to antibodies. Nonetheless, the fm1 mutant binds anti K(f) alloantisera and mAb much less strongly than the parent molecule, suggesting some indirect effect of these residues on serologic phenotype. The mutant is also interesting genetically because the sequence of the mutated region is identical to the sequence of the D(f) gene. This implies that there is a gene conversion-like mutational mechanism operating in the H-2(f) haplotype. Finally, the strategy used to obtain these K-locus cDNA should prove generally useful for isolating other MHC alleles.

Original languageEnglish (US)
Pages (from-to)1782-1787
Number of pages6
JournalJournal of Immunology
Volume145
Issue number6
StatePublished - Jan 1 1990

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Horton, R. M., Hildebrand, W. H., Martinko, J. M., & Pease, L. R. (1990). Structural analysis of H-2K(f) and H-2K(f)m1 by using H-2K locus-specific sequences. Journal of Immunology, 145(6), 1782-1787.