Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani

Daniel Sevlever, Peter Påhlsson, Graciela Rosen, Bo Nilsson, Mauricio V. Londner

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated from Leishmania donovani promastigotes. The carbohydrate moiety was cleaved from the lipid part by digestion with specific phosphatidylinositol phospholipase C. After separation, structural analysis was carried out on the phosphorylated inositol oligosaccharide and the alkylacyl glycerol. The following major structures were found: {Mathematical expression} The presence of the conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI of glycosyl phosphatidylinositol protein anchors in this antigen may be consistent with a precursor role of Leishmania glycosyl phosphatidylinositol anchored proteins for this glycolipid.

Original languageEnglish (US)
Pages (from-to)321-329
Number of pages9
JournalGlycoconjugate Journal
Volume8
Issue number4
DOIs
StatePublished - Aug 1991

Keywords

  • Leishmania donovani
  • antigen
  • glycolipid
  • glycosyl phosphatidylinositol
  • protein anchor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Sevlever, D., Påhlsson, P., Rosen, G., Nilsson, B., & Londner, M. V. (1991). Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani. Glycoconjugate Journal, 8(4), 321-329. https://doi.org/10.1007/BF00731344