Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani

Daniel Sevlever; Peter Påhlsson; Graciela Rosen; Bo Nilsson; Mauricio V. Londner

doi:10.1007/BF00731344

Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani

Daniel Sevlever, Peter Påhlsson, Graciela Rosen, Bo Nilsson, Mauricio V. Londner

Neuroscience

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated from Leishmania donovani promastigotes. The carbohydrate moiety was cleaved from the lipid part by digestion with specific phosphatidylinositol phospholipase C. After separation, structural analysis was carried out on the phosphorylated inositol oligosaccharide and the alkylacyl glycerol. The following major structures were found: {Mathematical expression} The presence of the conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI of glycosyl phosphatidylinositol protein anchors in this antigen may be consistent with a precursor role of Leishmania glycosyl phosphatidylinositol anchored proteins for this glycolipid.

Original language	English (US)
Pages (from-to)	321-329
Number of pages	9
Journal	Glycoconjugate Journal
Volume	8
Issue number	4
DOIs	https://doi.org/10.1007/BF00731344
State	Published - Aug 1991

Keywords

Leishmania donovani
antigen
glycolipid
glycosyl phosphatidylinositol
protein anchor

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1007/BF00731344

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title = "Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani",

abstract = "A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated from Leishmania donovani promastigotes. The carbohydrate moiety was cleaved from the lipid part by digestion with specific phosphatidylinositol phospholipase C. After separation, structural analysis was carried out on the phosphorylated inositol oligosaccharide and the alkylacyl glycerol. The following major structures were found: {Mathematical expression} The presence of the conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI of glycosyl phosphatidylinositol protein anchors in this antigen may be consistent with a precursor role of Leishmania glycosyl phosphatidylinositol anchored proteins for this glycolipid.",

keywords = "Leishmania donovani, antigen, glycolipid, glycosyl phosphatidylinositol, protein anchor",

author = "Daniel Sevlever and Peter P{\aa}hlsson and Graciela Rosen and Bo Nilsson and Londner, {Mauricio V.}",

year = "1991",

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language = "English (US)",

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T1 - Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani

AU - Sevlever, Daniel

AU - Påhlsson, Peter

AU - Rosen, Graciela

AU - Nilsson, Bo

AU - Londner, Mauricio V.

PY - 1991/8

Y1 - 1991/8

N2 - A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated from Leishmania donovani promastigotes. The carbohydrate moiety was cleaved from the lipid part by digestion with specific phosphatidylinositol phospholipase C. After separation, structural analysis was carried out on the phosphorylated inositol oligosaccharide and the alkylacyl glycerol. The following major structures were found: {Mathematical expression} The presence of the conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI of glycosyl phosphatidylinositol protein anchors in this antigen may be consistent with a precursor role of Leishmania glycosyl phosphatidylinositol anchored proteins for this glycolipid.

AB - A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated from Leishmania donovani promastigotes. The carbohydrate moiety was cleaved from the lipid part by digestion with specific phosphatidylinositol phospholipase C. After separation, structural analysis was carried out on the phosphorylated inositol oligosaccharide and the alkylacyl glycerol. The following major structures were found: {Mathematical expression} The presence of the conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI of glycosyl phosphatidylinositol protein anchors in this antigen may be consistent with a precursor role of Leishmania glycosyl phosphatidylinositol anchored proteins for this glycolipid.

KW - Leishmania donovani

KW - antigen

KW - glycolipid

KW - glycosyl phosphatidylinositol

KW - protein anchor

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Structural analysis of a glycosylphosphatidylinositol glycolipid of Leishmania donovani

Abstract

Keywords

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