Striking differences of LDL receptor-related protein 1B expression in mouse and human

Yonghe Li, Wenyan Lu, Guojun D Bu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The low density lipoprotein (LDL) receptor-related protein 1B (LRP1B) is a member of the expanding LDL receptor family, and is closely related to LRP. It was discovered as a putative tumor suppressor, and is frequently inactivated in human malignant tissues. However, the expression pattern of LRP1B in normal human tissues was unclear. In the present study, we analyzed LRP1B expression in normal mouse and human tissues. By using RT-PCR, we found that, while mouse LRP1B expression is mostly restricted to the brain, human LRP1B expression is more widespread with highest expression levels detected in the brain, adrenal gland, salivary gland, and testis. Although mouse LRP1B expresses in the forms of both full-length receptor tail and an alternatively spliced form lacking a 33-amino acid insert, human LRP1B is expressed exclusively in the form of full-length receptor tail. Finally, we found that, unlike mouse LRP1B, human LRP1B is cleaved by furin. Taken together, these data demonstrate that there are striking differences between LRP1B expression in mouse and human tissues. The broader expression pattern of LRP1B in human tissues suggests that this putative tumor suppressor may play roles in several types of human cancer.

Original languageEnglish (US)
Pages (from-to)868-873
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume333
Issue number3
DOIs
StatePublished - Aug 5 2005
Externally publishedYes

Fingerprint

LDL-Receptor Related Proteins
Low Density Lipoprotein Receptor-Related Protein-1
LDL Receptors
Proteins
Tissue
Tail
Tumors
Brain
Furin
Neoplasms
Adrenal Glands
Salivary Glands
Testis

Keywords

  • Alternative splicing
  • Expression pattern
  • Furin
  • LRP
  • LRP1B

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Striking differences of LDL receptor-related protein 1B expression in mouse and human. / Li, Yonghe; Lu, Wenyan; Bu, Guojun D.

In: Biochemical and Biophysical Research Communications, Vol. 333, No. 3, 05.08.2005, p. 868-873.

Research output: Contribution to journalArticle

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