Stretching type II collagen with optical tweezers

Yu Long Sun, Zong Ping Luo, Andrzej Fertala, Kai Nan An

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Collagens are the most abundant proteins of vertebrates and they provide mechanical and supportive functions in a wide range of connective tissues. Knowledge of the mechanical properties of single collagen molecules is essential in studying the self-assembly of collagen, the interaction between cells and extracellular matrix, the etiology of tissue degeneration and mechanism of regeneration, and the relationship between the structures and mechanical properties of tissues. Here we stretched single type II collagen molecules in neutral pH solution using optical tweezers. The molecular parameters of collagen were obtained by fitting force-extension curves into worm-like chain elasticity model. The molecule length of type II collagen monomer was 295.8nm. The persistence length of type II collagen monomer was 11.2nm. These observations indicate that collagen molecules are flexible rather than rigid rod molecules at neutral pH solution.

Original languageEnglish (US)
Pages (from-to)1665-1669
Number of pages5
JournalJournal of Biomechanics
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2004

Keywords

  • Flexibility
  • Optical tweezers
  • Persistence length
  • Type II collagen

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

Fingerprint Dive into the research topics of 'Stretching type II collagen with optical tweezers'. Together they form a unique fingerprint.

  • Cite this

    Sun, Y. L., Luo, Z. P., Fertala, A., & An, K. N. (2004). Stretching type II collagen with optical tweezers. Journal of Biomechanics, 37(11), 1665-1669. https://doi.org/10.1016/j.jbiomech.2004.02.028