Stretching short biopolymers using optical tweezers

Yu Long Sun; Zong Ping Luo; Kai Nan An

doi:10.1006/bbrc.2001.5426

Stretching short biopolymers using optical tweezers

Yu Long Sun, Zong Ping Luo, Kai Nan An

Orthopedic Surgery

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27 Scopus citations

Abstract

Optical tweezers is a useful technique to study single molecules. It has been applied to stretch biopolymers in several approaches. However, these approaches may not be appropriate to measure short biopolymers. In the present study, the optical signals of beads in different situations were studied. A new method was developed for stretching short biopolymers, as short as 300 nm in length. This new method was successfully applied to determine the stiffness of procollagen I molecules.

Original language	English (US)
Pages (from-to)	826-830
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	286
Issue number	4
DOIs	https://doi.org/10.1006/bbrc.2001.5426
State	Published - 2001

Keywords

Collagen
Optical tweezers
Short biopolymer
Single molecule

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.2001.5426

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title = "Stretching short biopolymers using optical tweezers",

abstract = "Optical tweezers is a useful technique to study single molecules. It has been applied to stretch biopolymers in several approaches. However, these approaches may not be appropriate to measure short biopolymers. In the present study, the optical signals of beads in different situations were studied. A new method was developed for stretching short biopolymers, as short as 300 nm in length. This new method was successfully applied to determine the stiffness of procollagen I molecules.",

keywords = "Collagen, Optical tweezers, Short biopolymer, Single molecule",

author = "Sun, {Yu Long} and Luo, {Zong Ping} and An, {Kai Nan}",

note = "Funding Information: This study was supported by grants from the National Institutes of Health (R01 AR44497), Arthritis Foundation, and Mayo Foundation. The authors thank A. Fertala for providing the procollagen sample.",

year = "2001",

doi = "10.1006/bbrc.2001.5426",

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T1 - Stretching short biopolymers using optical tweezers

AU - Sun, Yu Long

AU - Luo, Zong Ping

AU - An, Kai Nan

N1 - Funding Information: This study was supported by grants from the National Institutes of Health (R01 AR44497), Arthritis Foundation, and Mayo Foundation. The authors thank A. Fertala for providing the procollagen sample.

PY - 2001

Y1 - 2001

N2 - Optical tweezers is a useful technique to study single molecules. It has been applied to stretch biopolymers in several approaches. However, these approaches may not be appropriate to measure short biopolymers. In the present study, the optical signals of beads in different situations were studied. A new method was developed for stretching short biopolymers, as short as 300 nm in length. This new method was successfully applied to determine the stiffness of procollagen I molecules.

AB - Optical tweezers is a useful technique to study single molecules. It has been applied to stretch biopolymers in several approaches. However, these approaches may not be appropriate to measure short biopolymers. In the present study, the optical signals of beads in different situations were studied. A new method was developed for stretching short biopolymers, as short as 300 nm in length. This new method was successfully applied to determine the stiffness of procollagen I molecules.

KW - Collagen

KW - Optical tweezers

KW - Short biopolymer

KW - Single molecule

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Stretching short biopolymers using optical tweezers

Abstract

Keywords

ASJC Scopus subject areas

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