Stretch induces cytokine release by alveolar epithelial cells in vitro

Nicholas E. Vlahakis, Mark A. Schroeder, Andrew Harold Limper, Rolf D. Hubmayr

Research output: Contribution to journalArticle

328 Citations (Scopus)

Abstract

Mechanical ventilation can injure the lung, causing edema and alveolar inflammation. Interleukin-8 (IL-8) plays an important role in this inflammatory response. We postulated that cyclic cell stretch upregulates the production and release of IL-8 by human alveolar epithelium in the absence of structural cell damage or paracrine stimulation. To test this hypothesis, alveolar epithelial cells (A549 cells) were cultured on a deformable silicoelastic membrane. When stretched by 30% for up to 48 h, the cells released 49 ± 34% more IL-8 (P < 0.001) than static controls. Smaller deformations (20% stretch) produced no consistent increase in IL-8. Stretch of 4 h duration increased IL-8 gene transcription fourfold above baseline. Stretch had no effect on cell proliferation, cell viability as assessed by 51Cr release assay, or the release of granulocyte-macrophage colony- stimulating factor and tumor necrosis factor-α. We conclude that deformation per se can trigger inflammatory signaling and that alveolar epithelial cells may be active participants in the alveolitis associated with ventilator- induced lung injury.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume277
Issue number1 21-1
StatePublished - Jul 1999

Fingerprint

Alveolar Epithelial Cells
Interleukin-8
Cytokines
Ventilator-Induced Lung Injury
Granulocyte-Macrophage Colony-Stimulating Factor
Artificial Respiration
Edema
Cell Survival
Up-Regulation
Epithelium
Tumor Necrosis Factor-alpha
Cell Proliferation
In Vitro Techniques
Inflammation
Lung
Membranes
Genes

Keywords

  • Alveolar epithelium
  • Deformation
  • Inflammation
  • Interleukin-8
  • Mechanical forces

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Physiology (medical)
  • Pulmonary and Respiratory Medicine

Cite this

Stretch induces cytokine release by alveolar epithelial cells in vitro. / Vlahakis, Nicholas E.; Schroeder, Mark A.; Limper, Andrew Harold; Hubmayr, Rolf D.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 277, No. 1 21-1, 07.1999.

Research output: Contribution to journalArticle

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