Strengthening injectable thermo-sensitive NIPAAm-g-chitosan hydrogels using chemical cross-linking of disulfide bonds as scaffolds for tissue engineering

Shu Wei Wu, Xifeng Liu, A. Lee Miller, Yu Shiuan Cheng, Ming Long Yeh, Lichun Lu

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

In the present study, we fabricated non-toxic, injectable, and thermo-sensitive NIPAAm-g-chitosan (NC) hydrogels with thiol modification for introduction of disulfide cross-linking strategy. Previously, NIPAAm and chitosan copolymer has been proven to have excellent biocompatibility, biodegradability and rapid phase transition after injection, suitable to serve as cell carriers or implanted scaffolds. However, weak mechanical properties significantly limit their potential for biomedical fields. In order to overcome this issue, we incorporated thiol side chains into chitosan by covalently conjugating N-acetyl-cysteine (NAC) with carbodiimide chemistry to strengthen mechanical properties. After oxidation of thiols into disulfide bonds, modified NC hydrogels did improve the compressive modulus over 9 folds (11.4 kPa). Oscillatory frequency sweep showed a positive correlation between storage modulus and cross-liking density as well. Additionally, there was no cytotoxicity observed to mesenchymal stem cells, fibroblasts and osteoblasts. We suggested that the thiol-modified thermo-sensitive polysaccharide hydrogels are promising to be a cell-laden biomaterial for tissue regeneration.

Original languageEnglish (US)
Pages (from-to)308-316
Number of pages9
JournalCarbohydrate Polymers
Volume192
DOIs
StatePublished - Jul 15 2018

Keywords

  • Biomaterial mechanical property
  • Disulfide bonds
  • N-acetyl-cysteine
  • NIPAAm
  • Thermo-sensitive hydrogel
  • Thiolated chitosan

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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