Strategies for antigen loading of dendritic cells to enhance the antitumor immune response

Scott E. Strome, Stephen Voss, Ryan Wilcox, Tamekia L. Wakefield, Koji Tamada, Dallas Flies, Andrei Chapoval, Jun Lu, Jan Kasperbauer, Douglas Padley, Richard Geoffrey Vile, Dennis Gastineau, Peter Wettstein, Lieping Chen

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Abstract

Dendritic cells (DCs) primed with tumor antigens can effectively mediate the regression of a variety of established solid malignancies in both murine and human models. Despite such clinical efficacy, the optimal means of DC priming is unknown. The goal of this study was to compare three methods of tumor preparation: irradiation, boiling, or freeze thaw lysis for DC priming. Mouse bone marrow-derived DCs were loaded with defined ratios of E.G7 tumor cells expressing a model tumor antigen, OVA. Sensitized DCs were used for stimulation of OVA-specific CTLs derived from OT-1 T-cell receptor transgenic mice. IFN-γ release, determined by ELISA at 24 and 48 h, was used to assess the expression of antigens by DCs. DCs loaded with irradiated tumors were effective stimulators for OT-1 CTLs, whereas DCs stimulated with freeze-thawed or boiled tumors did not stimulate IFN-γ production. Freeze-thaw lysis appeared to inhibit CTL activity in vitro and in two of three cases, this effect was not overcome by the addition of OVA. The ability to load irradiated tumor cells was reproduced in two analogous human melanoma models using melanoma cell lines expressing gp100 and CTL clones specific for a gp100 melanoma antigen. Consistent with the in vitro data, only DC/irradiated tumor vaccines were effective in preventing or delaying outgrowth of E.G7 and a poorly immunogenic murine squamous cell carcinoma (SCCVII), on local tumor challenge. These data demonstrate that the method of tumor cell preparation clearly influences the ability of DCs to present antigen to T cells. Correlation of in vitro data with the generation of protective immunity in vivo suggests the utility of irradiated tumor-primed DCs as a means to generate protective immunity in patients with solid malignancies.

Original languageEnglish (US)
Pages (from-to)1884-1889
Number of pages6
JournalCancer Research
Volume62
Issue number6
StatePublished - Mar 15 2002

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Dendritic Cells
Antigens
Neoplasms
Neoplasm Antigens
gp100 Melanoma Antigen
Immunity
Melanoma
Cancer Vaccines
T-Cell Antigen Receptor
Tumor Burden
Transgenic Mice
Squamous Cell Carcinoma
Clone Cells
Bone Marrow
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Strome, S. E., Voss, S., Wilcox, R., Wakefield, T. L., Tamada, K., Flies, D., ... Chen, L. (2002). Strategies for antigen loading of dendritic cells to enhance the antitumor immune response. Cancer Research, 62(6), 1884-1889.

Strategies for antigen loading of dendritic cells to enhance the antitumor immune response. / Strome, Scott E.; Voss, Stephen; Wilcox, Ryan; Wakefield, Tamekia L.; Tamada, Koji; Flies, Dallas; Chapoval, Andrei; Lu, Jun; Kasperbauer, Jan; Padley, Douglas; Vile, Richard Geoffrey; Gastineau, Dennis; Wettstein, Peter; Chen, Lieping.

In: Cancer Research, Vol. 62, No. 6, 15.03.2002, p. 1884-1889.

Research output: Contribution to journalArticle

Strome, SE, Voss, S, Wilcox, R, Wakefield, TL, Tamada, K, Flies, D, Chapoval, A, Lu, J, Kasperbauer, J, Padley, D, Vile, RG, Gastineau, D, Wettstein, P & Chen, L 2002, 'Strategies for antigen loading of dendritic cells to enhance the antitumor immune response', Cancer Research, vol. 62, no. 6, pp. 1884-1889.
Strome SE, Voss S, Wilcox R, Wakefield TL, Tamada K, Flies D et al. Strategies for antigen loading of dendritic cells to enhance the antitumor immune response. Cancer Research. 2002 Mar 15;62(6):1884-1889.
Strome, Scott E. ; Voss, Stephen ; Wilcox, Ryan ; Wakefield, Tamekia L. ; Tamada, Koji ; Flies, Dallas ; Chapoval, Andrei ; Lu, Jun ; Kasperbauer, Jan ; Padley, Douglas ; Vile, Richard Geoffrey ; Gastineau, Dennis ; Wettstein, Peter ; Chen, Lieping. / Strategies for antigen loading of dendritic cells to enhance the antitumor immune response. In: Cancer Research. 2002 ; Vol. 62, No. 6. pp. 1884-1889.
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