Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks Stall

Chuanhe Yu, Haiyun Gan, Junhong Han, Zhi Xiong Zhou, Shaodong Jia, Andrei Chabes, Gianrico Farrugia, Tamas Ordog, Zhiguo Zhang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In eukaryotic cells, DNA replication proceeds with continuous synthesis of leading-strand DNA and discontinuous synthesis of lagging-strand DNA. Here we describe a method, eSPAN (enrichment and sequencing of protein-associated nascent DNA), which reveals the genome-wide association of proteins with leading and lagging strands of DNA replication forks. Using this approach in budding yeast, we confirm the strand specificities of DNA polymerases delta and epsilon and show that the PCNA clamp is enriched at lagging strands compared with leading-strand replication. Surprisingly, at stalled forks, PCNA is unloaded specifically from lagging strands. PCNA unloading depends onthe Elg1-containing alternative RFC complex, ubiquitination of PCNA, and the checkpoint kinases Mec1 and Rad53. Cells deficient in PCNA unloading exhibit increased chromosome breaks. Our studies provide a tool for studying replication-related processes and reveal a mechanism whereby checkpoint kinases regulate strand-specific unloading ofPCNA from stalled replication forks to maintain genome stability.

Original languageEnglish (US)
Pages (from-to)551-563
Number of pages13
JournalMolecular Cell
Volume56
Issue number4
DOIs
StatePublished - Nov 20 2014

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Proliferating Cell Nuclear Antigen
Protein Binding
DNA Replication
DNA
Phosphotransferases
DNA Polymerase II
DNA Polymerase III
Chromosome Breakage
Saccharomycetales
Genomic Instability
Ubiquitination
Protein Sequence Analysis
Eukaryotic Cells
Genome
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks Stall. / Yu, Chuanhe; Gan, Haiyun; Han, Junhong; Zhou, Zhi Xiong; Jia, Shaodong; Chabes, Andrei; Farrugia, Gianrico; Ordog, Tamas; Zhang, Zhiguo.

In: Molecular Cell, Vol. 56, No. 4, 20.11.2014, p. 551-563.

Research output: Contribution to journalArticle

Yu, Chuanhe ; Gan, Haiyun ; Han, Junhong ; Zhou, Zhi Xiong ; Jia, Shaodong ; Chabes, Andrei ; Farrugia, Gianrico ; Ordog, Tamas ; Zhang, Zhiguo. / Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks Stall. In: Molecular Cell. 2014 ; Vol. 56, No. 4. pp. 551-563.
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AU - Jia, Shaodong

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