Store-operated Ca2+ entry supports contractile function in hearts of hibernators

Olga V. Nakipova, Alexey S. Averin, Edward V. Evdokimovskii, Oleg Yu Pimenov, Leonid Kosarski, Dmitriy Ignat'ev, Andrey Anufriev, Yuri M. Kokoz, Santiago Reyes, Andre Terzic, Alexey E. Alekseev

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hibernators have a distinctive ability to adapt to seasonal changes of body temperature in a range between 37°C and near freezing, exhibiting, among other features, a unique reversibility of cardiac contractility. The adaptation of myocardial contractility in hibernation state relies on alterations of excitation contraction coupling, which becomes less-dependent from extracellular Ca2+ entry and is predominantly controlled by Ca2+ release from sarcoplasmic reticulum, replenished by the Ca2+-ATPase (SERCA). We found that the specific SERCA inhibitor cyclopiazonic acid (CPA), in contrast to its effect in papillary muscles (PM) from rat hearts, did not reduce but rather potentiated contractility of PM from hibernating ground squirrels (GS). In GS ventricles we identified drastically elevated, compared to rats, expression of Orai1, Stim1 and Trpc1/3/4/5/6/7 mRNAs, putative components of store operated Ca2+ channels (SOC). Trpc3 protein levels were found increased in winter compared to summer GS, yet levels of Trpc5, Trpc6 or Trpc7 remained unchanged. Under suppressed voltage-dependent K+, Na+ and Ca2+ currents, the SOC inhibitor 2-aminoethyl diphenylborinate (2-APB) diminished whole-cell membrane currents in isolated cardiomyocytes from hibernating GS, but not from rats. During cooling-reheating cycles (30°C-7°C-30°C) of ground squirrel PM, 2-APB did not affect typical CPA-sensitive elevation of contractile force at low temperatures, but precluded the contractility at 30°C before and after the cooling. Wash-out of 2-APB reversed PM contractility to control values. Thus, we suggest that SOC play a pivotal role in governing the ability of hibernator hearts to maintain their function during the transition in and out of hibernating states.

Original languageEnglish (US)
Article numbere0177469
JournalPLoS One
Volume12
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Sciuridae
Papillary Muscles
squirrels
heart
Muscle
calcium
Rats
Calcium-Transporting ATPases
cyclopiazonic acid
Sarcoplasmic Reticulum
muscle strength
sarcoplasmic reticulum
Ca2-transporting ATPase
Body Temperature Changes
rats
Cooling
cooling
Hibernation
Excitation Contraction Coupling
body temperature changes

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Nakipova, O. V., Averin, A. S., Evdokimovskii, E. V., Pimenov, O. Y., Kosarski, L., Ignat'ev, D., ... Alekseev, A. E. (2017). Store-operated Ca2+ entry supports contractile function in hearts of hibernators. PLoS One, 12(5), [e0177469]. https://doi.org/10.1371/journal.pone.0177469

Store-operated Ca2+ entry supports contractile function in hearts of hibernators. / Nakipova, Olga V.; Averin, Alexey S.; Evdokimovskii, Edward V.; Pimenov, Oleg Yu; Kosarski, Leonid; Ignat'ev, Dmitriy; Anufriev, Andrey; Kokoz, Yuri M.; Reyes, Santiago; Terzic, Andre; Alekseev, Alexey E.

In: PLoS One, Vol. 12, No. 5, e0177469, 01.05.2017.

Research output: Contribution to journalArticle

Nakipova, OV, Averin, AS, Evdokimovskii, EV, Pimenov, OY, Kosarski, L, Ignat'ev, D, Anufriev, A, Kokoz, YM, Reyes, S, Terzic, A & Alekseev, AE 2017, 'Store-operated Ca2+ entry supports contractile function in hearts of hibernators', PLoS One, vol. 12, no. 5, e0177469. https://doi.org/10.1371/journal.pone.0177469
Nakipova OV, Averin AS, Evdokimovskii EV, Pimenov OY, Kosarski L, Ignat'ev D et al. Store-operated Ca2+ entry supports contractile function in hearts of hibernators. PLoS One. 2017 May 1;12(5). e0177469. https://doi.org/10.1371/journal.pone.0177469
Nakipova, Olga V. ; Averin, Alexey S. ; Evdokimovskii, Edward V. ; Pimenov, Oleg Yu ; Kosarski, Leonid ; Ignat'ev, Dmitriy ; Anufriev, Andrey ; Kokoz, Yuri M. ; Reyes, Santiago ; Terzic, Andre ; Alekseev, Alexey E. / Store-operated Ca2+ entry supports contractile function in hearts of hibernators. In: PLoS One. 2017 ; Vol. 12, No. 5.
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