Abstract
Background: Molecular changes associated with colorectal cancer (CRC) are detected by stool deoxyribonucleic acid testing but could persist following tumor resection. Aims: We sought to determine whether methylated gene markers in stool normalize after CRC resection. Methods: We studied stools from 22 CRC cases before and after subtotal resection and from 80 colonoscopy-normal controls. In blinded fashion, target genes (methylated NDRG4 and BMP3) were captured from stool supernatant, bisulfite-treated, and assayed by quantitative allele-specific real-time target and signal amplification. Results were dichotomized at 95 % specificity cutoffs. Results: Among CRC cases, median methylated NDRG4 and BMP3 levels decreased dramatically (4- to 15-fold) following resection, p = 0.003 and p < 0.0001, respectively. Among the 14 cases with elevated preoperative levels, 13 (93 %) fell into the normal range after surgery, p = 0.0002. A case whose stool methylated NDRG4 level increased sharply after surgery was found to have recurrent CRC. Conclusions: Methylated gene marker levels clear from stool following CRC resection unless disease is present. Postoperative stool marker levels are informative and may be of value in surveillance.
Original language | English (US) |
---|---|
Pages (from-to) | 1764-1767 |
Number of pages | 4 |
Journal | Digestive diseases and sciences |
Volume | 59 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2014 |
Keywords
- Colorectal neoplasms
- DNA methylation
- Feces analysis
- Secondary prevention
ASJC Scopus subject areas
- Physiology
- Gastroenterology