Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy

Yingxue Ren, Daniel L. Vera, Kimberly A. Hughes, Jonathan H. Dennis

Abstract

In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4. h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.

Original languageEnglish (US)
Pages (from-to)146-147
Number of pages2
JournalGenomics Data
Volume3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Drosophila
  • Illumina Hi-Seq
  • MNase
  • Nucleosome
  • Salmonella

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

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