Abstract
In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4. h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.
Original language | English (US) |
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Pages (from-to) | 146-147 |
Number of pages | 2 |
Journal | Genomics Data |
Volume | 3 |
DOIs | |
State | Published - Mar 1 2015 |
Keywords
- Drosophila
- Illumina Hi-Seq
- MNase
- Nucleosome
- Salmonella
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Medicine
- Genetics