Stimulation of secretion from bovine adrenal chromaffin cells by microsecond bursts of therapeutic levels of ultrasound

I. M. Robinson, R. R. Kinnick, J. F. Greenleaf, J. M. Fernandez

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

1. In this study the secretory response of individual bovine adrenal chromaffin cells was monitored using amperometric carbon-fibre microelectrodes. Cells were stimulated to secrete by exposure to 20-100 μs long tonehursts of ultrasonnd (2-4 x 105 Pa; peak pressure at 1 MHz). 2. Three types of secretory responses were observed: an almost instantaneous response, a delayed release of catecholamines, or a series of 'burst-like' secretory bouts. 3. Fura-2 measurements of intracellular Ca2+ concentrations showed that the release of catecholamines was accompanied by an increase in the intracellular Ca2+ concentration. In the absence of extracellular Ca2+, secretory responses were not evoked showing that Ca2+ entry was necessary to elicit catecholamine release.

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalJournal of Physiology
Volume492
Issue number1
DOIs
StatePublished - Apr 1 1996

ASJC Scopus subject areas

  • Physiology

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