Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration

Heather M. Argadine, Mark E. Bolander, James F Greenleaf

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Pulsed ultrasound has become a common therapy for delayed unions and nonunions after fractures. Experiments using ATDC5 cells, a mouse clonal chondrogenic cell line, have shown that 1 kHz dynamic acoustic radiation force stimulates proteoglycan synthesis similar to 1.5 MHz pulsed ultrasound. The results of two experiments performed 8 months apart showed that chondrocytes treated with 1 kHz squarewave had a 2 to 4-fold (p<0.01) increase in total area of nodules compared to control. When the same experiments were repeated 2 months later with the same frozen cell stock, the cells were unresponsive to mechanical stimulation, suggesting the cell line had transformed and was unable to differentiate into chondrocytes. This study demonstrates the need for primary chondrocytes to further examine the biological effects of 1 kHz vibration.

Original languageEnglish (US)
Title of host publicationProceedings - IEEE Ultrasonics Symposium
Pages849-851
Number of pages3
Volume1
DOIs
StatePublished - 2006

Fingerprint

stimulation
cultured cells
vibration
synthesis
cells
unions
biological effects
nodules
sound waves
mice
therapy

Keywords

  • 1 kHz
  • ATDC5
  • Chondrocyte
  • Chondrogenesis

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

Cite this

Argadine, H. M., Bolander, M. E., & Greenleaf, J. F. (2006). Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration. In Proceedings - IEEE Ultrasonics Symposium (Vol. 1, pp. 849-851). [4152077] https://doi.org/10.1109/ULTSYM.2006.221

Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration. / Argadine, Heather M.; Bolander, Mark E.; Greenleaf, James F.

Proceedings - IEEE Ultrasonics Symposium. Vol. 1 2006. p. 849-851 4152077.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Argadine, HM, Bolander, ME & Greenleaf, JF 2006, Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration. in Proceedings - IEEE Ultrasonics Symposium. vol. 1, 4152077, pp. 849-851. https://doi.org/10.1109/ULTSYM.2006.221
Argadine HM, Bolander ME, Greenleaf JF. Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration. In Proceedings - IEEE Ultrasonics Symposium. Vol. 1. 2006. p. 849-851. 4152077 https://doi.org/10.1109/ULTSYM.2006.221
Argadine, Heather M. ; Bolander, Mark E. ; Greenleaf, James F. / Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration. Proceedings - IEEE Ultrasonics Symposium. Vol. 1 2006. pp. 849-851
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