Stimulation of neurite outgrowth using positively charged hydrogels

Mahrokh Dadsetan, Andrew M. Knight, Lichun Lu, Anthony John Windebank, Michael J Yaszemski

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Autologous nerve grafts are currently the best option for the treatment of segmental peripheral nerve defects. However, autografts have several drawbacks including size mismatch and loss of sensation in the donor nerve's sensory distribution. In this work, we have investigated the development of a synthetic hydrogel that contains positive charge for use as a substrate for nerve cell attachment and neurite outgrowth in culture. We have demonstrated that modification of oligo-(polyethylene glycol) fumarate (OPF) with a positively charged monomer improves primary sensory rat neuron attachment and differentiation in a dose-dependent manner. Positively charged hydrogels also supported attachment of dorsal root ganglion (DRG) explants that contain sensory neurons, Schwann cells and neuronal support cells. Furthermore, charged hydrogels were analyzed for the appearance of myelinated structures in a co-culture containing DRG neurons and Schwann cells. DRGs and Schwann cells remained viable on charged hydrogels for a time period of three weeks and neurites extended from the DRGs. Sudan black staining revealed that neurites emerging from DRGs were accompanied by migrating Schwann cells. These findings suggest that charged OPF hydrogels are capable of sustaining both primary nerve cells and the neural support cells that are critical for regeneration.

Original languageEnglish (US)
Pages (from-to)3874-3881
Number of pages8
JournalBiomaterials
Volume30
Issue number23-24
DOIs
StatePublished - Aug 2009

Fingerprint

Hydrogels
Schwann Cells
Neurons
Diagnosis-Related Groups
Cells
Spinal Ganglia
Sensory Receptor Cells
Neurites
Fumarates
Sudan
Hydrogel
Autografts
Coculture Techniques
Peripheral Nerves
Regeneration
Cell culture
Grafts
Polyethylene glycols
Rats
Staining and Labeling

Keywords

  • Hydrogel
  • Nerve regeneration
  • Scaffold
  • Schwann cells

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Stimulation of neurite outgrowth using positively charged hydrogels. / Dadsetan, Mahrokh; Knight, Andrew M.; Lu, Lichun; Windebank, Anthony John; Yaszemski, Michael J.

In: Biomaterials, Vol. 30, No. 23-24, 08.2009, p. 3874-3881.

Research output: Contribution to journalArticle

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