Stimulation of glycosaminoglycan accumulation by interferon gamma in cultured human retroocular fibroblasts

Terry J. Smith, Rebecca S. Bahn, Colum A. Gorman, Michael Cheavens

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Fully expressed Graves' disease involves an accumulation of glycosaminoglycan (GAG) in the retroocular connective tissue which contributes to the pathogensis of ophthalmopathy. We treated cultured retroocular and dermal fibroblasts with recombinant interferon gamma (100 U/ml) for 16-24h and measured [3H]GAG accumulation. The cytokine stimulated [3H]GAG accumulation in retroocular fibroblast cultures obtained from eight different donors by 36-124% above control values. In contrast, interferon gamma had no consistent effect on macromolecular accumulation in dermal fibroblast cultures derived from the pretibium or from areas ordinarily uninvolved in Graves' dermopathy. These results suggest that retroocular fibroblasts may be uniquely targeted for one action of interferon gamma which involves the modulation of GAG metabolism.

Original languageEnglish (US)
Pages (from-to)1169-1171
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume72
Issue number5
StatePublished - May 1991

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Fibroblasts
Glycosaminoglycans
Interferon-gamma
Cell culture
Skin
Graves Disease
Metabolism
Connective Tissue
Modulation
Tissue
Cytokines

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Stimulation of glycosaminoglycan accumulation by interferon gamma in cultured human retroocular fibroblasts. / Smith, Terry J.; Bahn, Rebecca S.; Gorman, Colum A.; Cheavens, Michael.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 72, No. 5, 05.1991, p. 1169-1171.

Research output: Contribution to journalArticle

Smith, Terry J. ; Bahn, Rebecca S. ; Gorman, Colum A. ; Cheavens, Michael. / Stimulation of glycosaminoglycan accumulation by interferon gamma in cultured human retroocular fibroblasts. In: Journal of Clinical Endocrinology and Metabolism. 1991 ; Vol. 72, No. 5. pp. 1169-1171.
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