Stimulation of glycosaminoglycan accumulation by interferon gamma in cultured human retroocular fibroblasts

Terry J. Smith, Rebecca S. Bahn, Colum A. Gorman, Michael Cheavens

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Fully expressed Graves’ disease involves an accumulation of glycosaminoglycan (GAG) in the retroocular connective tissue which contributes to the pathogensis of ophthalmopathy. We treated cultured retroocular and dermal fibroblasts with recombinant interferon gamma (100 U/ml) for 16 – 24h and measured [3H]GAG accumulation. The cytokine stimulated [3H]GAG accumulation in retroocular fibroblast cultures obtained from eight different donors by 36–124% above control values. In contrast, interferon gamma had no consistent effect on macromolecular accumulation in dermal fibroblast cultures derived from the pretibium or from areas ordinarily uninvolved in Graves’ dermopathy. These results suggest that retroocular fibroblasts may be uniquely targeted for one action of interferon gamma which involves the modulation of GAG metabolism.

Original languageEnglish (US)
Pages (from-to)1169-1171
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume72
Issue number5
DOIs
StatePublished - Apr 1991

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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