Stereospecific determination and in vivo monodeiodination of thyroxine enantiomers in euthyroid man

Ian D. Hay; Colum A. Gorman; Kenneth D. Burman; Nai Siang Jiang

doi:10.1016/0026-0495(85)90011-3

Stereospecific determination and in vivo monodeiodination of thyroxine enantiomers in euthyroid man

Ian D. Hay, Colum A. Gorman, Kenneth D. Burman, Nai Siang Jiang

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

To compare in man the absorption, serum disappearance, and peripheral monodeiodination of the thyroxine enantiomers, we studied six euthyroid subjects who, on separate occasions, orally ingested 3 mg of either dextrothyroxine (DT₄) or levothyroxine (LT₄). We measured the serum concentrations of total T₄ (TT₄), total T₃, and reverse T₃ (rT₃) by nonstereospecific radioimmunoassay and we determined serum DT₄ by stereospecific chromatography. Mean serum TT₄ levels from 4 hours were significantly greater after LT₄ administration. After DT₄ administration, stereospecific analysis of serum revealed two T₄ peaks that persisted from 2 to 48 hours. The mean serum LT₄ leved did not significantly change during the 48 hours after DT₄ administration. Increases in serum T₃ and rT₃ were seen from 2 hours after administration of either enantiomer. From 12 hours the levels of both triiodothyronines after LT₄ were significantly higher than after DT₄. In this short term study we found no evidence that in man DT₄ is converted to LT₄, nor is it preferentially deiodinated to rT₃. The greater and more persistent increases in serum T₄ and T₃ observed after LT₄ probably contribute to the known higher bioactivity of that enantiomer.

Original language	English (US)
Pages (from-to)	266-271
Number of pages	6
Journal	Metabolism
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/0026-0495(85)90011-3
State	Published - Mar 1985

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1016/0026-0495(85)90011-3

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@article{968a508c3f0c42d0b5c54dde0f3cba4b,

title = "Stereospecific determination and in vivo monodeiodination of thyroxine enantiomers in euthyroid man",

abstract = "To compare in man the absorption, serum disappearance, and peripheral monodeiodination of the thyroxine enantiomers, we studied six euthyroid subjects who, on separate occasions, orally ingested 3 mg of either dextrothyroxine (DT4) or levothyroxine (LT4). We measured the serum concentrations of total T4 (TT4), total T3, and reverse T3 (rT3) by nonstereospecific radioimmunoassay and we determined serum DT4 by stereospecific chromatography. Mean serum TT4 levels from 4 hours were significantly greater after LT4 administration. After DT4 administration, stereospecific analysis of serum revealed two T4 peaks that persisted from 2 to 48 hours. The mean serum LT4 leved did not significantly change during the 48 hours after DT4 administration. Increases in serum T3 and rT3 were seen from 2 hours after administration of either enantiomer. From 12 hours the levels of both triiodothyronines after LT4 were significantly higher than after DT4. In this short term study we found no evidence that in man DT4 is converted to LT4, nor is it preferentially deiodinated to rT3. The greater and more persistent increases in serum T4 and T3 observed after LT4 probably contribute to the known higher bioactivity of that enantiomer.",

author = "Hay, {Ian D.} and Gorman, {Colum A.} and Burman, {Kenneth D.} and Jiang, {Nai Siang}",

note = "Funding Information: From the Division of Endocrinology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, and the Walter Reed Army Medical Center, Washington, DC. Presented in part at the 57th Annual Meeting of the American Thyroid Association, Minneapolis, September i 98 I. Supported in part by a grant from Travenol Laboratories, Inc. Deerfield, III. The opinions or assertions contained herein are the private views of the authors and are not to be construed as ojkial or as reflecting the views of the Department of the Army or the Department of Defense. Address reprint requests to Dr Ian D. Hay, Division of Endocrinology and Internal Medicine, Mayo Clinic, Rochester, MN 5590s. D I985 by Grune & Stratton, Inc. 0026-0495/8S/3403-0010$03.00/0",

year = "1985",

month = mar,

doi = "10.1016/0026-0495(85)90011-3",

language = "English (US)",

volume = "34",

pages = "266--271",

journal = "Metabolism",

issn = "0026-0495",

publisher = "W.B. Saunders Ltd",

number = "3",

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T1 - Stereospecific determination and in vivo monodeiodination of thyroxine enantiomers in euthyroid man

AU - Hay, Ian D.

AU - Gorman, Colum A.

AU - Burman, Kenneth D.

AU - Jiang, Nai Siang

N1 - Funding Information: From the Division of Endocrinology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, and the Walter Reed Army Medical Center, Washington, DC. Presented in part at the 57th Annual Meeting of the American Thyroid Association, Minneapolis, September i 98 I. Supported in part by a grant from Travenol Laboratories, Inc. Deerfield, III. The opinions or assertions contained herein are the private views of the authors and are not to be construed as ojkial or as reflecting the views of the Department of the Army or the Department of Defense. Address reprint requests to Dr Ian D. Hay, Division of Endocrinology and Internal Medicine, Mayo Clinic, Rochester, MN 5590s. D I985 by Grune & Stratton, Inc. 0026-0495/8S/3403-0010$03.00/0

PY - 1985/3

Y1 - 1985/3

N2 - To compare in man the absorption, serum disappearance, and peripheral monodeiodination of the thyroxine enantiomers, we studied six euthyroid subjects who, on separate occasions, orally ingested 3 mg of either dextrothyroxine (DT4) or levothyroxine (LT4). We measured the serum concentrations of total T4 (TT4), total T3, and reverse T3 (rT3) by nonstereospecific radioimmunoassay and we determined serum DT4 by stereospecific chromatography. Mean serum TT4 levels from 4 hours were significantly greater after LT4 administration. After DT4 administration, stereospecific analysis of serum revealed two T4 peaks that persisted from 2 to 48 hours. The mean serum LT4 leved did not significantly change during the 48 hours after DT4 administration. Increases in serum T3 and rT3 were seen from 2 hours after administration of either enantiomer. From 12 hours the levels of both triiodothyronines after LT4 were significantly higher than after DT4. In this short term study we found no evidence that in man DT4 is converted to LT4, nor is it preferentially deiodinated to rT3. The greater and more persistent increases in serum T4 and T3 observed after LT4 probably contribute to the known higher bioactivity of that enantiomer.

AB - To compare in man the absorption, serum disappearance, and peripheral monodeiodination of the thyroxine enantiomers, we studied six euthyroid subjects who, on separate occasions, orally ingested 3 mg of either dextrothyroxine (DT4) or levothyroxine (LT4). We measured the serum concentrations of total T4 (TT4), total T3, and reverse T3 (rT3) by nonstereospecific radioimmunoassay and we determined serum DT4 by stereospecific chromatography. Mean serum TT4 levels from 4 hours were significantly greater after LT4 administration. After DT4 administration, stereospecific analysis of serum revealed two T4 peaks that persisted from 2 to 48 hours. The mean serum LT4 leved did not significantly change during the 48 hours after DT4 administration. Increases in serum T3 and rT3 were seen from 2 hours after administration of either enantiomer. From 12 hours the levels of both triiodothyronines after LT4 were significantly higher than after DT4. In this short term study we found no evidence that in man DT4 is converted to LT4, nor is it preferentially deiodinated to rT3. The greater and more persistent increases in serum T4 and T3 observed after LT4 probably contribute to the known higher bioactivity of that enantiomer.

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VL - 34

SP - 266

EP - 271

JO - Metabolism

JF - Metabolism

IS - 3

ER -

Stereospecific determination and in vivo monodeiodination of thyroxine enantiomers in euthyroid man

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