Stem Cell−Laden Coaxially Electrospun Fibrous Scaffold for Regenerative Engineering Applications

Naveen Nagiah, Federico Franchi, Karen Peterson, Martin Rodriguez-Porcel

Research output: Contribution to journalArticlepeer-review

Abstract

Stem cell−based therapies for various ailments have attracted significant attention for over a decade. However, low retention of transplanted cells at the damaged site has hindered their potential for use in therapy. Tissue engineered grafts with fibrillar structures mimicking the extracellular matrix (ECM) can be potentially used to increase the retention and engraftment of stem cells at the damaged site. Moreover, these grafts may also provide mechanical stability at the damaged site to enhance function and regeneration. Among all the methods to produce fibrillar structures developed in recent years, electrospinning is a simple and versatile method to produce fibrous structures ranging from a few nanometers to micrometers. Coaxial electrospinning enables production of a mechanically stable core with a cell-binding sheath for enhanced cell adhesion and proliferation. Furthermore, this process provides an alternative to functionalized engineered scaffolds with specific compositions. The present article describes the protocol for developing a polycaprolactone (PCL) core and gelatin/gelatin methacrylate (GelMA) sheath laden with stem cells for various regenerative engineering applications.

Original languageEnglish (US)
Article numbere13
JournalCurrent Protocols
Volume1
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • coaxial
  • electrospinning
  • gelatin
  • GelMA
  • PCL
  • stem cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Health Informatics
  • Medical Laboratory Technology

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