Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo

Thomas R. Ulich, Juan Del Castillo, Ian K. McNiece, Eunhee S. Yi, Concepcion P. Alzona, Songmei Yin, Krisztina M. Zsebo

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Recombinant rat stem cell factor (rrSCF) and recombinant human granulocyte colony-stimulating factor (G-GSF) coinjected for 1 week in rats cause a synergistic increase in mature marrow neutrophils accompanied by a striking decrease in erythroid and lymphoid marrow elements. The spleens of the same rats show increased granulopoiesis as well as increased erythropoiesis as compared with the spleens of rats treated with either growth factor alone. Splenic extramedullary erythropoiesis may act to compensate for the decrease in marrow erythropoiesis. The coinjection of rrSCF and G-CSF causes an increase in marrow mast cells at the end of 1 week, but the increase is much less than in rrSCF-alone-treated rats. The combination of rrSCF and G-CSF increases the rate of release of marrow neutrophils into the circulation and causes a dramatic synergistic peripheral neutrophilia, beginning especially after 4 days of treatment. Colony-forming assays of all experimental groups showed a synergistic increase in colony-forming unit granulecyte-macrophage (CFU-GM) in the marrow, but not in peripheral blood, after coincubation with SCF plus granulocyte-macrophage CSF (GM-CSF) as opposed to GM-CSF alone, showing anatomic compartmentalization between a more primitive marrow CFU-GM subset and a more mature peripheral blood CFU-GM subset. In vivo daily administration of SCF plus GM-CSF results in a synergistic increase in marrow neutrophils, but not the striking synergistic increase in circulating neutrophils that is observed with SCF plus G-CSF.

Original languageEnglish (US)
Pages (from-to)1954-1962
Number of pages9
JournalBlood
Volume78
Issue number8
StatePublished - Oct 15 1991
Externally publishedYes

Fingerprint

Stem Cell Factor
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Rats
Bone Marrow
Macrophages
Colony-Stimulating Factors
Erythropoiesis
Neutrophils
Stem Cells
Granulocytes
Blood
Spleen
Mast Cells
Assays
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Ulich, T. R., Del Castillo, J., McNiece, I. K., Yi, E. S., Alzona, C. P., Yin, S., & Zsebo, K. M. (1991). Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo. Blood, 78(8), 1954-1962.

Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo. / Ulich, Thomas R.; Del Castillo, Juan; McNiece, Ian K.; Yi, Eunhee S.; Alzona, Concepcion P.; Yin, Songmei; Zsebo, Krisztina M.

In: Blood, Vol. 78, No. 8, 15.10.1991, p. 1954-1962.

Research output: Contribution to journalArticle

Ulich, TR, Del Castillo, J, McNiece, IK, Yi, ES, Alzona, CP, Yin, S & Zsebo, KM 1991, 'Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo', Blood, vol. 78, no. 8, pp. 1954-1962.
Ulich, Thomas R. ; Del Castillo, Juan ; McNiece, Ian K. ; Yi, Eunhee S. ; Alzona, Concepcion P. ; Yin, Songmei ; Zsebo, Krisztina M. / Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo. In: Blood. 1991 ; Vol. 78, No. 8. pp. 1954-1962.
@article{e8a85eaaea3f44b7bfde0347f857e899,
title = "Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo",
abstract = "Recombinant rat stem cell factor (rrSCF) and recombinant human granulocyte colony-stimulating factor (G-GSF) coinjected for 1 week in rats cause a synergistic increase in mature marrow neutrophils accompanied by a striking decrease in erythroid and lymphoid marrow elements. The spleens of the same rats show increased granulopoiesis as well as increased erythropoiesis as compared with the spleens of rats treated with either growth factor alone. Splenic extramedullary erythropoiesis may act to compensate for the decrease in marrow erythropoiesis. The coinjection of rrSCF and G-CSF causes an increase in marrow mast cells at the end of 1 week, but the increase is much less than in rrSCF-alone-treated rats. The combination of rrSCF and G-CSF increases the rate of release of marrow neutrophils into the circulation and causes a dramatic synergistic peripheral neutrophilia, beginning especially after 4 days of treatment. Colony-forming assays of all experimental groups showed a synergistic increase in colony-forming unit granulecyte-macrophage (CFU-GM) in the marrow, but not in peripheral blood, after coincubation with SCF plus granulocyte-macrophage CSF (GM-CSF) as opposed to GM-CSF alone, showing anatomic compartmentalization between a more primitive marrow CFU-GM subset and a more mature peripheral blood CFU-GM subset. In vivo daily administration of SCF plus GM-CSF results in a synergistic increase in marrow neutrophils, but not the striking synergistic increase in circulating neutrophils that is observed with SCF plus G-CSF.",
author = "Ulich, {Thomas R.} and {Del Castillo}, Juan and McNiece, {Ian K.} and Yi, {Eunhee S.} and Alzona, {Concepcion P.} and Songmei Yin and Zsebo, {Krisztina M.}",
year = "1991",
month = "10",
day = "15",
language = "English (US)",
volume = "78",
pages = "1954--1962",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "8",

}

TY - JOUR

T1 - Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoicsis in vivo

AU - Ulich, Thomas R.

AU - Del Castillo, Juan

AU - McNiece, Ian K.

AU - Yi, Eunhee S.

AU - Alzona, Concepcion P.

AU - Yin, Songmei

AU - Zsebo, Krisztina M.

PY - 1991/10/15

Y1 - 1991/10/15

N2 - Recombinant rat stem cell factor (rrSCF) and recombinant human granulocyte colony-stimulating factor (G-GSF) coinjected for 1 week in rats cause a synergistic increase in mature marrow neutrophils accompanied by a striking decrease in erythroid and lymphoid marrow elements. The spleens of the same rats show increased granulopoiesis as well as increased erythropoiesis as compared with the spleens of rats treated with either growth factor alone. Splenic extramedullary erythropoiesis may act to compensate for the decrease in marrow erythropoiesis. The coinjection of rrSCF and G-CSF causes an increase in marrow mast cells at the end of 1 week, but the increase is much less than in rrSCF-alone-treated rats. The combination of rrSCF and G-CSF increases the rate of release of marrow neutrophils into the circulation and causes a dramatic synergistic peripheral neutrophilia, beginning especially after 4 days of treatment. Colony-forming assays of all experimental groups showed a synergistic increase in colony-forming unit granulecyte-macrophage (CFU-GM) in the marrow, but not in peripheral blood, after coincubation with SCF plus granulocyte-macrophage CSF (GM-CSF) as opposed to GM-CSF alone, showing anatomic compartmentalization between a more primitive marrow CFU-GM subset and a more mature peripheral blood CFU-GM subset. In vivo daily administration of SCF plus GM-CSF results in a synergistic increase in marrow neutrophils, but not the striking synergistic increase in circulating neutrophils that is observed with SCF plus G-CSF.

AB - Recombinant rat stem cell factor (rrSCF) and recombinant human granulocyte colony-stimulating factor (G-GSF) coinjected for 1 week in rats cause a synergistic increase in mature marrow neutrophils accompanied by a striking decrease in erythroid and lymphoid marrow elements. The spleens of the same rats show increased granulopoiesis as well as increased erythropoiesis as compared with the spleens of rats treated with either growth factor alone. Splenic extramedullary erythropoiesis may act to compensate for the decrease in marrow erythropoiesis. The coinjection of rrSCF and G-CSF causes an increase in marrow mast cells at the end of 1 week, but the increase is much less than in rrSCF-alone-treated rats. The combination of rrSCF and G-CSF increases the rate of release of marrow neutrophils into the circulation and causes a dramatic synergistic peripheral neutrophilia, beginning especially after 4 days of treatment. Colony-forming assays of all experimental groups showed a synergistic increase in colony-forming unit granulecyte-macrophage (CFU-GM) in the marrow, but not in peripheral blood, after coincubation with SCF plus granulocyte-macrophage CSF (GM-CSF) as opposed to GM-CSF alone, showing anatomic compartmentalization between a more primitive marrow CFU-GM subset and a more mature peripheral blood CFU-GM subset. In vivo daily administration of SCF plus GM-CSF results in a synergistic increase in marrow neutrophils, but not the striking synergistic increase in circulating neutrophils that is observed with SCF plus G-CSF.

UR - http://www.scopus.com/inward/record.url?scp=0025986571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025986571&partnerID=8YFLogxK

M3 - Article

VL - 78

SP - 1954

EP - 1962

JO - Blood

JF - Blood

SN - 0006-4971

IS - 8

ER -