Stealth technology: How Epstein-Barr virus utilizes DNA methylation to cloak itself from immune detection

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is a large lymphotrophic DNA virus that establishes life-long residency in the infected host and is associated with a number of human tumors. The EBV genome encodes proteins essential for persistence, an oncoprotein, and proteins that render it vulnerable to the host's immune system; therefore, EBV gene transcription is tightly regulated. One critically important regulatory mechanism utilized by EBV is DNA methylation. Methylation of cytosines within CpG dinucleotides at promoter regions is important for gene silencing and genome integrity. Although most parasitic elements are methylated in mammalian cells never to be reactivated again, EBV has evolved to utilize DNA methylation to maximize persistence and cloak itself from immune detection. EBV's reliance on DNA methylation also provides a unique therapeutic strategy for the treatment of EBV-associated tumors. DNA demethylating agents are capable of reactivating transcription of highly immunogenic viral proteins, rendering tumor cells susceptible to killing by the host immune system, and inducing the viral lytic cycle which culminates in cell lysis.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalClinical Immunology
Volume109
Issue number1
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

DNA Methylation
Human Herpesvirus 4
Technology
Immune System
Genome
Neoplasms
DNA Viruses
Oncogene Proteins
Cytosine
Gene Silencing
Viral Proteins
Internship and Residency
Genetic Promoter Regions
Methylation
Proteins
DNA
Genes

Keywords

  • Bisulfite genomic sequencing
  • DNA methylation
  • EBV
  • Epstein-Barr virus
  • ICF syndrome
  • Latent
  • Lytic
  • Transcriptional regulation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Stealth technology : How Epstein-Barr virus utilizes DNA methylation to cloak itself from immune detection. / Tao, Qian; Robertson, Keith D.

In: Clinical Immunology, Vol. 109, No. 1, 10.2003, p. 53-63.

Research output: Contribution to journalArticle

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