STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus

Yongyao Fu; Jocelyn Wang; Gayathri Panangipalli; Benjamin J. Ulrich; Byunghee Koh; Chengxian Xu; Rakshin Kharwadkar; Xiaona Chu; Yue Wang; Hongyu Gao; Wenting Wu; Jie Sun; Robert S. Tepper; Baohua Zhou; Sarath Chandra Janga; Kai Yang; Mark H. Kaplan

doi:10.1038/s41467-020-18648-6

STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus

Yongyao Fu, Jocelyn Wang, Gayathri Panangipalli, Benjamin J. Ulrich, Byunghee Koh, Chengxian Xu, Rakshin Kharwadkar, Xiaona Chu, Yue Wang, Hongyu Gao, Wenting Wu, Jie Sun, Robert S. Tepper, Baohua Zhou, Sarath Chandra Janga, Kai Yang, Mark H. Kaplan

Pulmonary and Critical Care Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

T helper cell differentiation requires lineage-defining transcription factors and factors that have shared expression among multiple subsets. BATF is required for development of multiple Th subsets but functions in a lineage-specific manner. BATF is required for IL-9 production in Th9 cells but in contrast to its function as a pioneer factor in Th17 cells, BATF is neither sufficient nor required for accessibility at the Il9 locus. Here we show that STAT5 is the earliest factor binding and remodeling the Il9 locus to allow BATF binding in both mouse and human Th9 cultures. The ability of STAT5 to mediate accessibility for BATF is observed in other Th lineages and allows acquisition of the IL-9-secreting phenotype. STAT5 and BATF convert Th17 cells into cells that mediate IL-9-dependent effects in allergic airway inflammation and anti-tumor immunity. Thus, BATF requires the STAT5 signal to mediate plasticity at the Il9 locus.

Original language	English (US)
Article number	4882
Journal	Nature communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-18648-6
State	Published - Dec 1 2020

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Fu, Y., Wang, J., Panangipalli, G., Ulrich, B. J., Koh, B., Xu, C., Kharwadkar, R., Chu, X., Wang, Y., Gao, H., Wu, W., Sun, J., Tepper, R. S., Zhou, B., Janga, S. C., Yang, K., & Kaplan, M. H. (2020). STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus. Nature communications, 11(1), Article 4882. https://doi.org/10.1038/s41467-020-18648-6

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title = "STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus",

abstract = "T helper cell differentiation requires lineage-defining transcription factors and factors that have shared expression among multiple subsets. BATF is required for development of multiple Th subsets but functions in a lineage-specific manner. BATF is required for IL-9 production in Th9 cells but in contrast to its function as a pioneer factor in Th17 cells, BATF is neither sufficient nor required for accessibility at the Il9 locus. Here we show that STAT5 is the earliest factor binding and remodeling the Il9 locus to allow BATF binding in both mouse and human Th9 cultures. The ability of STAT5 to mediate accessibility for BATF is observed in other Th lineages and allows acquisition of the IL-9-secreting phenotype. STAT5 and BATF convert Th17 cells into cells that mediate IL-9-dependent effects in allergic airway inflammation and anti-tumor immunity. Thus, BATF requires the STAT5 signal to mediate plasticity at the Il9 locus.",

author = "Yongyao Fu and Jocelyn Wang and Gayathri Panangipalli and Ulrich, {Benjamin J.} and Byunghee Koh and Chengxian Xu and Rakshin Kharwadkar and Xiaona Chu and Yue Wang and Hongyu Gao and Wenting Wu and Jie Sun and Tepper, {Robert S.} and Baohua Zhou and Janga, {Sarath Chandra} and Kai Yang and Kaplan, {Mark H.}",

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doi = "10.1038/s41467-020-18648-6",

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AU - Fu, Yongyao

AU - Wang, Jocelyn

AU - Panangipalli, Gayathri

AU - Ulrich, Benjamin J.

AU - Koh, Byunghee

AU - Xu, Chengxian

AU - Kharwadkar, Rakshin

AU - Chu, Xiaona

AU - Wang, Yue

AU - Gao, Hongyu

AU - Wu, Wenting

AU - Sun, Jie

AU - Tepper, Robert S.

AU - Zhou, Baohua

AU - Janga, Sarath Chandra

AU - Yang, Kai

AU - Kaplan, Mark H.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - T helper cell differentiation requires lineage-defining transcription factors and factors that have shared expression among multiple subsets. BATF is required for development of multiple Th subsets but functions in a lineage-specific manner. BATF is required for IL-9 production in Th9 cells but in contrast to its function as a pioneer factor in Th17 cells, BATF is neither sufficient nor required for accessibility at the Il9 locus. Here we show that STAT5 is the earliest factor binding and remodeling the Il9 locus to allow BATF binding in both mouse and human Th9 cultures. The ability of STAT5 to mediate accessibility for BATF is observed in other Th lineages and allows acquisition of the IL-9-secreting phenotype. STAT5 and BATF convert Th17 cells into cells that mediate IL-9-dependent effects in allergic airway inflammation and anti-tumor immunity. Thus, BATF requires the STAT5 signal to mediate plasticity at the Il9 locus.

AB - T helper cell differentiation requires lineage-defining transcription factors and factors that have shared expression among multiple subsets. BATF is required for development of multiple Th subsets but functions in a lineage-specific manner. BATF is required for IL-9 production in Th9 cells but in contrast to its function as a pioneer factor in Th17 cells, BATF is neither sufficient nor required for accessibility at the Il9 locus. Here we show that STAT5 is the earliest factor binding and remodeling the Il9 locus to allow BATF binding in both mouse and human Th9 cultures. The ability of STAT5 to mediate accessibility for BATF is observed in other Th lineages and allows acquisition of the IL-9-secreting phenotype. STAT5 and BATF convert Th17 cells into cells that mediate IL-9-dependent effects in allergic airway inflammation and anti-tumor immunity. Thus, BATF requires the STAT5 signal to mediate plasticity at the Il9 locus.

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STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus

Abstract

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