Staining with fluorescein diacetate correlates with hepatocyte function

Scott Nyberg, Russell A. Shatford, William D. Payne, Wei Shou Hu, Frank B. Cerra

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalBiotechnic and Histochemistry
Volume68
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Hepatocytes
Fluorescence
Staining and Labeling
Lidocaine
3',6'-diacetylfluorescein
Albumins
Flow Cytometry
Coloring Agents

Keywords

  • Albumin production
  • Flow cytometry
  • Fluorescein diacetate
  • Hepatocyte
  • Lidocaine metabolism. viability

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Cite this

Staining with fluorescein diacetate correlates with hepatocyte function. / Nyberg, Scott; Shatford, Russell A.; Payne, William D.; Hu, Wei Shou; Cerra, Frank B.

In: Biotechnic and Histochemistry, Vol. 68, No. 1, 01.01.1993, p. 56-63.

Research output: Contribution to journalArticle

Nyberg, Scott ; Shatford, Russell A. ; Payne, William D. ; Hu, Wei Shou ; Cerra, Frank B. / Staining with fluorescein diacetate correlates with hepatocyte function. In: Biotechnic and Histochemistry. 1993 ; Vol. 68, No. 1. pp. 56-63.
@article{a1f97df803eb4dd0af88177190b02067,
title = "Staining with fluorescein diacetate correlates with hepatocyte function",
abstract = "To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.",
keywords = "Albumin production, Flow cytometry, Fluorescein diacetate, Hepatocyte, Lidocaine metabolism. viability",
author = "Scott Nyberg and Shatford, {Russell A.} and Payne, {William D.} and Hu, {Wei Shou} and Cerra, {Frank B.}",
year = "1993",
month = "1",
day = "1",
doi = "10.3109/10520299309105579",
language = "English (US)",
volume = "68",
pages = "56--63",
journal = "Biotechnic and Histochemistry",
issn = "1052-0295",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Staining with fluorescein diacetate correlates with hepatocyte function

AU - Nyberg, Scott

AU - Shatford, Russell A.

AU - Payne, William D.

AU - Hu, Wei Shou

AU - Cerra, Frank B.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.

AB - To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.

KW - Albumin production

KW - Flow cytometry

KW - Fluorescein diacetate

KW - Hepatocyte

KW - Lidocaine metabolism. viability

UR - http://www.scopus.com/inward/record.url?scp=0027468401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027468401&partnerID=8YFLogxK

U2 - 10.3109/10520299309105579

DO - 10.3109/10520299309105579

M3 - Article

C2 - 7680583

AN - SCOPUS:0027468401

VL - 68

SP - 56

EP - 63

JO - Biotechnic and Histochemistry

JF - Biotechnic and Histochemistry

SN - 1052-0295

IS - 1

ER -