Staining with fluorescein diacetate correlates with hepatocyte function

Scott L. Nyberg, Russell A. Shatford, William D. Payne, Wei Shou Hu, Frank B. Cerra

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2 = 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalBiotechnic and Histochemistry
Volume68
Issue number1
DOIs
StatePublished - Jan 1 1993

Keywords

  • Albumin production
  • Flow cytometry
  • Fluorescein diacetate
  • Hepatocyte
  • Lidocaine metabolism. viability

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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