Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells

K. A. Haines, Thomas J Flotte, T. A. Springer, I. Gigli, G. J. Thorbecke

Research output: Contribution to journalArticle

40 Scopus citations


Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and -3, Ia antigen, Fc fragment receptor, and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationships of Langerhans cells to the interdigitating cells of lymphoid tissues.

Original languageEnglish (US)
Pages (from-to)3448-3451
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number11 I
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

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