Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells

K. A. Haines, Thomas J Flotte, T. A. Springer, I. Gigli, G. J. Thorbecke

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and -3, Ia antigen, Fc fragment receptor, and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationships of Langerhans cells to the interdigitating cells of lymphoid tissues.

Original languageEnglish (US)
Pages (from-to)3448-3451
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number11 I
StatePublished - 1983
Externally publishedYes

Fingerprint

Langerhans Cells
Macrophages
Monoclonal Antibodies
Lymphocytes
Dendritic Cells
Staining and Labeling
Histocompatibility Antigens Class II
Lymphoid Tissue
Follicular Dendritic Cells
CD45 Antigens
Galectin 3
Immunoglobulin Fc Fragments
Fc Receptors
Antigen-Presenting Cells
Surface Antigens
Epidermis
Suspensions
Antigens

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells. / Haines, K. A.; Flotte, Thomas J; Springer, T. A.; Gigli, I.; Thorbecke, G. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, No. 11 I, 1983, p. 3448-3451.

Research output: Contribution to journalArticle

@article{28f92e846f254393b4d9a27ba0e76328,
title = "Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells",
abstract = "Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and -3, Ia antigen, Fc fragment receptor, and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationships of Langerhans cells to the interdigitating cells of lymphoid tissues.",
author = "Haines, {K. A.} and Flotte, {Thomas J} and Springer, {T. A.} and I. Gigli and Thorbecke, {G. J.}",
year = "1983",
language = "English (US)",
volume = "80",
pages = "3448--3451",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11 I",

}

TY - JOUR

T1 - Staining of Langerhans cells with monoclonal antibodies to macrophages and lymphoid cells

AU - Haines, K. A.

AU - Flotte, Thomas J

AU - Springer, T. A.

AU - Gigli, I.

AU - Thorbecke, G. J.

PY - 1983

Y1 - 1983

N2 - Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and -3, Ia antigen, Fc fragment receptor, and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationships of Langerhans cells to the interdigitating cells of lymphoid tissues.

AB - Langerhans cells are Ia-bearing antigen-presenting cells in the epidermis that share many functions with macrophages. We have used monoclonal antibodies to the macrophage antigens, Mac-2 and -3, Ia antigen, Fc fragment receptor, and the common leukocyte antigen CLA to compare the cell surface antigens of these cells with those of interdigitating and follicular dendritic cells and of macrophages in lymphoid tissues. Immunoperoxidase staining was carried out with epidermal sheets from BALB/c mice and epidermal cell suspensions enriched for Langerhans cells by Fc rosetting. Langerhans cells stained for all of these antigens. Comparison with the staining properties of other dendritic cells and macrophages, in combination with previous observations, indicates a close relationships of Langerhans cells to the interdigitating cells of lymphoid tissues.

UR - http://www.scopus.com/inward/record.url?scp=0020565817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020565817&partnerID=8YFLogxK

M3 - Article

C2 - 6574493

AN - SCOPUS:0020565817

VL - 80

SP - 3448

EP - 3451

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11 I

ER -