TY - JOUR
T1 - Stable transfection of UCP1 confers resistance to hypoxia/reoxygenation in a heart-derived cell line
AU - Bienengraeber, Martin
AU - Ozcan, Cevher
AU - Terzic, Andre
N1 - Funding Information:
This work was supported by National Institutes of Health, American Heart Association, Miami Heart Research Institute, Marriott Foundation and Rappaport Program in Vascular Biology and Gene Delivery. A.T. is an Established Investigator of the American Heart Association.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Mitochondrial uncoupling proteins, which secure physiological uncoupling of oxidative phosphorylation, have been proposed to serve as an oxidative-stress compensatory mechanism. Here, heart-derived H9c2 cells acquired improved resistance to injury upon transfection of the prototypic uncoupling protein UCP1. Following hypoxia/reoxygenation, stable overexpression of UCP1 provided enhanced cardioblast survival with preserved mitochondrial structure and function, while limiting reactive oxygen species formation. Thus, transfection of mitochondrial UCP1 provides a strategy for generation of a stress-resistant cardiac cell phenotype.
AB - Mitochondrial uncoupling proteins, which secure physiological uncoupling of oxidative phosphorylation, have been proposed to serve as an oxidative-stress compensatory mechanism. Here, heart-derived H9c2 cells acquired improved resistance to injury upon transfection of the prototypic uncoupling protein UCP1. Following hypoxia/reoxygenation, stable overexpression of UCP1 provided enhanced cardioblast survival with preserved mitochondrial structure and function, while limiting reactive oxygen species formation. Thus, transfection of mitochondrial UCP1 provides a strategy for generation of a stress-resistant cardiac cell phenotype.
KW - Cardioprotection
KW - Mitochondria
KW - Reactive oxygen species
KW - Uncoupling protein
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U2 - 10.1016/S0022-2828(03)00147-0
DO - 10.1016/S0022-2828(03)00147-0
M3 - Article
C2 - 12818577
AN - SCOPUS:0038123011
SN - 0022-2828
VL - 35
SP - 861
EP - 865
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 7
ER -