Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.

Medicine & Life Sciences

• Isotope Labeling 100%
• Proteomics 62%
• Cell Culture Techniques 52%
• Amino Acids 47%
• Leucine 35%
• Proteins 25%
• Glyceraldehyde-3-Phosphate Dehydrogenases 14%
• Essential Amino Acids 14%
• Pyruvate Kinase 14%
• Population Control 13%
• Cell Line 13%
• Electrophoresis, Gel, Two-Dimensional 13%
• Complex Mixtures 12%
• Isotopes 12%
• Growth 11%
• Fibronectins 11%
• Muscle Cells 10%
• Cell Differentiation 9%
• Peptides 7%

Chemical Compounds

• Stable Isotope 74%
• Protein 28%
• Leucine 23%
• Acid 23%
• Glyceraldehyde 3-Phosphate 15%
• Fibronectin 13%
• Two Dimensional Polyacrylamide Gel Electrophoresis 13%
• Pyruvate 11%
• Mammalian Cell 11%
• Peptide 6%
• Mixture 4%
• Time 4%