Stabilized β-catenin immortalizes colonic epithelial cells

R. A. Wagenaar, H. C. Crawford, L. M. Matrisian

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The majority of colonic neoplasias contain mutations in either the adenomatous polyposis coli or the β-catenin (β-cat) gene, both of which result in elevated levels of cytoplasmic β-cat. The oncogenic activity of β-cat has been explored in vivo and in vitro with conflicting results. We tested the hypothesis that β-cat is capable of immortalizing and transforming cultured epithelial cells that represent the precursors to colon cancer. A truncated form of β-cat (ΔN89) was stably expressed in murine colonic epithelial cells that were conditionally immortalized by temperature-sensitive T antigen expression and contained a mutant ApcMin allele [Immorto-Min colonic epithelium (IMCE)]. IMCE cells, grown under nonpermissive conditions, were immortalized by expression of the truncated β-cat protein as determined by sustained growth in culture and escape from senescence as measured by endogenous β-galactosidase activity. IMCE neo cells at nonpermissive conditions underwent extensive apoptosis, an effect that was blocked by the expression of ΔN89β-catenin. IMCE β-cat cells had significantly lower p19 and p53 protein levels compared to IMCE neo cells, suggesting that alterations in these two key genes may mediate the effects of β-cat on both cellular senescence and apoptosis. IMCE β-cat cells were also transformed as determined by growth in the absence of serum, anchorage-independent growth, and sustained tumor growth in nude mice. Stable β-cat-expressing populations could not be generated in conditionally immortalized colonic epithelial cells with a wild-type Apc background. These studies demonstrated the immortalizing activity of stabilized β-cat for the first time and extend the transforming ability of mutated β-cat to a cell line representing a precursor to colorectal cancer.

Original languageEnglish (US)
Pages (from-to)2097-2104
Number of pages8
JournalCancer Research
Volume61
Issue number5
StatePublished - Mar 1 2001
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wagenaar, R. A., Crawford, H. C., & Matrisian, L. M. (2001). Stabilized β-catenin immortalizes colonic epithelial cells. Cancer Research, 61(5), 2097-2104.