Stabilization of measles virus for vaccine formulation

Julian Kissmann, Salvador F. Ausar, Angela Rudolph, Chad Braun, Stephen P. Cape, Robert E. Sievers, Mark J. Federspiel, Sangeeta B. Joshi, C. Russell Middaugh

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

An attenuated live measles virus (MV) was characterized by several biophysical methods as a function of temperature and pH. Following a method developed previously, the resultant light scattering and spectroscopic data were synthesized into an empirical phase diagram that visually and simultaneously represents the entire data set. Using this empirically-based phase diagram, screening assays were developed to identify potential vaccine stabilizers. Various compounds are shown by these assays to inhibit the temperature-induced aggregation of viral particles, and also to protect the integrity of the viral envelope. Accelerated stability assays show that, upon thermal challenge, MV formulated with these excipients retains its infectivity to a significant extent. Thus, the enhanced physical stability produced by this method is shown to protect the biological activity of this important but labile vaccine.

Original languageEnglish (US)
Pages (from-to)350-359
Number of pages10
JournalHuman Vaccines
Volume4
Issue number5
DOIs
StatePublished - Jan 1 2008

Keywords

  • Circular dichroism
  • Empirical phase diagram
  • Fluorescence
  • Formulation
  • Light scattering
  • Measles
  • Stability
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Kissmann, J., Ausar, S. F., Rudolph, A., Braun, C., Cape, S. P., Sievers, R. E., Federspiel, M. J., Joshi, S. B., & Middaugh, C. R. (2008). Stabilization of measles virus for vaccine formulation. Human Vaccines, 4(5), 350-359. https://doi.org/10.4161/hv.4.5.5863