Stabilities of intrastrand pyrimidine motif DNA and RNA triple helices

Paula Rodrigues Hoyne, A. Marquis Gacy, Cynthia T. McMurray, L. James Maher

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Nucleic acid triple helices have provoked interest since their discovery more than 40 years ago, but it remains unknown whether such structures occur naturally in cells. To pursue this question, it is important to determine the stabilities of representative triple helices at physiological temperature and pH. Previous investigations have concluded that while both DNA and RNA can participate in the pyrimidine tripler motif under mildly acidic conditions, these structures are often relatively unstable at neutral pH. We are now exploring the stability of intrastrand DNA and RNA pyrimidine motif triplexes at physiological temperature and pH. Duplex and tripler formation were monitored by thermal denaturation analysis, circular dichroism spectroscopy and gel shift experiments. Short intrastrand triplexes were observed to form in the pyrimidine motif in both DNA and RNA. In the presence of physiological concentrations of Mg2+ and at physiological pH, all detected triplexes were sufficiently stable to persist at physiological temperature. If sequences specifying such intrastrand triplexes are encoded in genomes, the potential exists for the formation of stable structures in RNA or DNA in vivo.

Original languageEnglish (US)
Pages (from-to)770-775
Number of pages6
JournalNucleic acids research
Volume28
Issue number3
StatePublished - Feb 1 2000

ASJC Scopus subject areas

  • Genetics

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    Rodrigues Hoyne, P., Gacy, A. M., McMurray, C. T., & Maher, L. J. (2000). Stabilities of intrastrand pyrimidine motif DNA and RNA triple helices. Nucleic acids research, 28(3), 770-775.