Src-mediated tyrosine phosphorylation of Protein Kinase D2 at focal adhesions regulates cell adhesion

Nisha Durand, Ligia I. Bastea, Heike Döppler, Tim Eiseler, Peter Storz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dependent on their cellular localization, Protein Kinase D (PKD) enzymes regulate different processes including Golgi transport, cell signaling and response to oxidative stress. The localization of PKD within cells is mediated by interaction with different lipid or protein binding partners. With the example of PKD2, we here show that phosphorylation events can also contribute to localization of subcellular pools of this kinase. Specifically, in the present study, we show that tyrosine phosphorylation of PKD2 at residue Y87 defines its localization to the focal adhesions and leads to activation. This phosphorylation occurs downstream of RhoA signaling and is mediated via Src. Moreover, mutation of this residue blocks PKD2's interaction with Focal Adhesion Kinase (FAK). The presence and regulation of PKD2 at focal adhesions identifies a novel function for this kinase as a modulator of cell adhesion and migration.

Original languageEnglish (US)
Article number9524
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Focal Adhesions
Cell Adhesion
Protein-Tyrosine Kinases
Phosphorylation
4 alpha-glucanotransferase
Phosphotransferases
Focal Adhesion Protein-Tyrosine Kinases
Protein Binding
Cell Movement
Tyrosine
Oxidative Stress
Lipids
Mutation
protein kinase D2
protein kinase D

ASJC Scopus subject areas

  • General

Cite this

Src-mediated tyrosine phosphorylation of Protein Kinase D2 at focal adhesions regulates cell adhesion. / Durand, Nisha; Bastea, Ligia I.; Döppler, Heike; Eiseler, Tim; Storz, Peter.

In: Scientific Reports, Vol. 7, No. 1, 9524, 01.12.2017.

Research output: Contribution to journalArticle

Durand, Nisha ; Bastea, Ligia I. ; Döppler, Heike ; Eiseler, Tim ; Storz, Peter. / Src-mediated tyrosine phosphorylation of Protein Kinase D2 at focal adhesions regulates cell adhesion. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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