Src-mediated phosphorylation of dynamin and cortactin regulates the "constitutive" endocytosis of transferrin

Hong Cao, Jing Chen, Eugene W. Krueger, Mark A. McNiven

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The mechanisms by which epithelial cells regulate clathrin-mediated endocytosis (CME) of transferrin are poorly defined and generally viewed as a constitutive process that occurs continuously without regulatory constraints. In this study, we demonstrate for the first time that endocytosis of the transferrin receptor is a regulated process that requires activated Src kinase and, subsequently, phosphorylation of two important components of the endocytic machinery, namely, the large GTPase dynamin 2 (Dyn2) and its associated actin-binding protein, cortactin (Cort). To our knowledge these findings are among the first to implicate an Src-mediated endocytic cascade in what was previously presumed to be a nonregulated internalization process.

Original languageEnglish (US)
Pages (from-to)781-792
Number of pages12
JournalMolecular and cellular biology
Volume30
Issue number3
DOIs
StatePublished - Feb 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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