TY - JOUR
T1 - Src-mediated phosphorylation of dynamin and cortactin regulates the "constitutive" endocytosis of transferrin
AU - Cao, Hong
AU - Chen, Jing
AU - Krueger, Eugene W.
AU - McNiven, Mark A.
PY - 2010/2
Y1 - 2010/2
N2 - The mechanisms by which epithelial cells regulate clathrin-mediated endocytosis (CME) of transferrin are poorly defined and generally viewed as a constitutive process that occurs continuously without regulatory constraints. In this study, we demonstrate for the first time that endocytosis of the transferrin receptor is a regulated process that requires activated Src kinase and, subsequently, phosphorylation of two important components of the endocytic machinery, namely, the large GTPase dynamin 2 (Dyn2) and its associated actin-binding protein, cortactin (Cort). To our knowledge these findings are among the first to implicate an Src-mediated endocytic cascade in what was previously presumed to be a nonregulated internalization process.
AB - The mechanisms by which epithelial cells regulate clathrin-mediated endocytosis (CME) of transferrin are poorly defined and generally viewed as a constitutive process that occurs continuously without regulatory constraints. In this study, we demonstrate for the first time that endocytosis of the transferrin receptor is a regulated process that requires activated Src kinase and, subsequently, phosphorylation of two important components of the endocytic machinery, namely, the large GTPase dynamin 2 (Dyn2) and its associated actin-binding protein, cortactin (Cort). To our knowledge these findings are among the first to implicate an Src-mediated endocytic cascade in what was previously presumed to be a nonregulated internalization process.
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U2 - 10.1128/MCB.00330-09
DO - 10.1128/MCB.00330-09
M3 - Article
C2 - 19995918
AN - SCOPUS:75149163629
SN - 0270-7306
VL - 30
SP - 781
EP - 792
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 3
ER -