Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2

Shaun G. Weller, Mirco Capitani, Hong Cao, Massimo Micaroni, Alberto Luini, Michele Sallese, Mark A Mc Niven

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The size and integrity of the Golgi apparatus is maintained via a tightly controlled regulation of membrane traffic using a variety of different signaling and cytoskeletal proteins. We have recently observed that activation of c-Src has profound effects on Golgi structure, leadingto dramatically vesiculatedcisternae in a variety of cell types. As the large GTPase dynamin (Dyn2) has been implicated in Golgi vesiculation during secretion, we tested whether inhibiting Dyn2 activity by expression of a Dyn2K44A mutant or siRNA knockdown could attenuate active Src-induced Golgi fragmentation. Indeed, these perturbations attenuated fragmentation, and expression of a Dyn2Y(231/597)F mutant protein that cannot be phosphorylated by Src kinase had a similar effect . Finally, we find that Dyn2 is markedly phosphorylated during the transit of VSV-G protein through the TGNwhereas expression of the Dyn2Y(231/597) F mutant significantly reduces exit of the nascent protein from this compartment. These findings demonstrate that activation of Dyn2 by Src kinase regulates Golgi integrity and vesiculation during the secretory process.

Original languageEnglish (US)
Pages (from-to)5863-5868
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number13
DOIs
StatePublished - Mar 30 2010

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Dynamin II
src-Family Kinases
Golgi Apparatus
Dynamins
Cytoskeletal Proteins
Secretory Pathway
GTP Phosphohydrolases
Mutant Proteins
GTP-Binding Proteins
Small Interfering RNA
Membranes
Proteins

Keywords

  • Dynamin
  • Golgi vesiculation

ASJC Scopus subject areas

  • General

Cite this

Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2. / Weller, Shaun G.; Capitani, Mirco; Cao, Hong; Micaroni, Massimo; Luini, Alberto; Sallese, Michele; Mc Niven, Mark A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 13, 30.03.2010, p. 5863-5868.

Research output: Contribution to journalArticle

Weller, Shaun G. ; Capitani, Mirco ; Cao, Hong ; Micaroni, Massimo ; Luini, Alberto ; Sallese, Michele ; Mc Niven, Mark A. / Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 13. pp. 5863-5868.
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AU - Luini, Alberto

AU - Sallese, Michele

AU - Mc Niven, Mark A

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