Spontaneous vulvar papillomas in a colony of mice used for pancreatic cancer research

Naomi M. Gades, Akihiro Ohashi, Lisa D. Mills, Matt A. Rowley, Kelly S. Predmore, Ronald J. Marler, Fergus J. Couch

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Abstract

Mice in a colony used for pancreatic cancer research and maintained in a barrier animal facility presented with vulvar masses. A census and examination of all colony animals was conducted on 17 February 2006; line, gender, and mass location were recorded; a slide caliper was used to measure the width, length, and height of each mass; and the volume of each mass was calculated. Progeny female mice from crossbreeding of the B6.FVB-Tg(Ipf1-cre)1Tuv and B6;129-Kras2tm4Tyj (KRASG12D/+) strains presented with external vulvar and periauricular papillomas. The papillomas were present in 41.2% of all female crossbred mice and ranged in size from 8 to 36 mm3. Age of mice and tumor size were not correlated. Compared with the B6.FVB-Tg(Ipf1-cre) 1Tuv line, the crossbred female mice were more likely to have a vulvar mass, with an odds ratio of 29.3, 95% confidence interval (1.5, 563.9) and a positive predictive value of 42.9%. Diagnostic evaluation, including electron microscopy, light microscopy, serology, and bacteriology, did not reveal a viral or other infectious etiology. Therefore, we speculate that interaction between the genetic background of the mice and the introduced Kras oncogene may be responsible for these papillomas.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalComparative medicine
Volume58
Issue number3
StatePublished - Jun 1 2008

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Gades, N. M., Ohashi, A., Mills, L. D., Rowley, M. A., Predmore, K. S., Marler, R. J., & Couch, F. J. (2008). Spontaneous vulvar papillomas in a colony of mice used for pancreatic cancer research. Comparative medicine, 58(3), 271-275.