Spontaneous fusion of cells between species yields transdifferentiation and retroviral transfer in vivo.

Brenda M. Ogle, Kim A. Butters, Timothy B. Plummer, Kevin R. Ring, Bruce E. Knudsen, Mark R Litzow, Marilia Cascalho, Jeffrey L. Platt

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Human cells can fuse with damaged or diseased somatic cells in vivo. Whether human cells fuse in vivo in the absence of disease and with cells of disparate species is unknown. Such a question is of current interest because blood exchanges between species through direct physical contact, via insect vectors or parasitism, are thought to underlie the transmission of zoonotic agents. In a model of human-pig chimerism, we show that some human hematopoietic stem cells engrafted in pigs contain both human and porcine chromosomal DNA. These hybrid cells divide, express human and porcine proteins, and contribute to porcine nonhematopoietic tissues. In addition, the hybrid cells contain porcine endogenous retroviral DNA sequences and are able to transmit this virus to uninfected human cells in vitro. Thus, spontaneous fusion can occur in vivo between the cells of disparate species and in the absence of disease. The ability of these cell hybrids to acquire and transmit retroviral elements together with their ability to integrate into tissues could explain genetic recombination and generation of novel pathogens. * differentiation * fusion * retrovirus

Original languageEnglish (US)
Pages (from-to)548-550
Number of pages3
JournalThe FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume18
Issue number3
StatePublished - 2004

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Cell Fusion
Fusion reactions
Cells
Electric fuses
Swine
Hybrid Cells
Tissue
DNA sequences
Pathogens
Stem cells
Viruses
Blood
Insect Vectors
DNA
Chimerism
Zoonoses
Retroviridae
Hematopoietic Stem Cells
Proteins
Genetic Recombination

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Spontaneous fusion of cells between species yields transdifferentiation and retroviral transfer in vivo. / Ogle, Brenda M.; Butters, Kim A.; Plummer, Timothy B.; Ring, Kevin R.; Knudsen, Bruce E.; Litzow, Mark R; Cascalho, Marilia; Platt, Jeffrey L.

In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, No. 3, 2004, p. 548-550.

Research output: Contribution to journalArticle

Ogle, Brenda M. ; Butters, Kim A. ; Plummer, Timothy B. ; Ring, Kevin R. ; Knudsen, Bruce E. ; Litzow, Mark R ; Cascalho, Marilia ; Platt, Jeffrey L. / Spontaneous fusion of cells between species yields transdifferentiation and retroviral transfer in vivo. In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2004 ; Vol. 18, No. 3. pp. 548-550.
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