Spontaneous calcium waves without contraction in cardiac myocytes

José R. López, Aleksandar Jovanovic, Andre Terzic

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.

Original languageEnglish (US)
Pages (from-to)781-787
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number3
DOIs
StatePublished - 1995

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Calcium Signaling
Cardiac Myocytes
Calcium
Confocal Microscopy
Wave propagation
Sarcomeres
Fluorescent Dyes
Muscle Cells
Confocal microscopy
Lasers

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Spontaneous calcium waves without contraction in cardiac myocytes. / López, José R.; Jovanovic, Aleksandar; Terzic, Andre.

In: Biochemical and Biophysical Research Communications, Vol. 214, No. 3, 1995, p. 781-787.

Research output: Contribution to journalArticle

López, José R. ; Jovanovic, Aleksandar ; Terzic, Andre. / Spontaneous calcium waves without contraction in cardiac myocytes. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 214, No. 3. pp. 781-787.
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