Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1

Yan Xu; Kui Zhu; Guiying Hong; Weihua Wu; Linnea M. Baudhuin; Yi Jin Xiao; Derek S. Damron

doi:10.1038/35010529

Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1

Yan Xu, Kui Zhu, Guiying Hong, Weihua Wu, Linnea M. Baudhuin, Yi Jin Xiao, Derek S. Damron

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

169 Scopus citations

Abstract

Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to be mediated by the activation of unidentified G-protein-coupled receptors. Here we sinew that SPC is a high-affinity ligand for an orphan receptor, ovarian cancer G-protein-coupled receptor 1 (OGR1). In OGR1-transfected cells, SPC binds to OGR1 with high affinity (K(d) = 33.3 nM) and high specificity and transiently increases intracellular calcium. The specific binding of SPC to OGR1 also activates p42/44 mitogen-activated protein kinases (MAP kinases) and inhibits cell proliferation. In addition, SPC causes internalization of OGR1 in a structurally specific manner.

Original language	English (US)
Pages (from-to)	261-267
Number of pages	7
Journal	Nature Cell Biology
Volume	2
Issue number	5
DOIs	https://doi.org/10.1038/35010529
State	Published - 2000

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1038/35010529

Cite this

@article{427e29e10c814ef19a0c9e62eac56892,

title = "Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1",

abstract = "Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to be mediated by the activation of unidentified G-protein-coupled receptors. Here we sinew that SPC is a high-affinity ligand for an orphan receptor, ovarian cancer G-protein-coupled receptor 1 (OGR1). In OGR1-transfected cells, SPC binds to OGR1 with high affinity (K(d) = 33.3 nM) and high specificity and transiently increases intracellular calcium. The specific binding of SPC to OGR1 also activates p42/44 mitogen-activated protein kinases (MAP kinases) and inhibits cell proliferation. In addition, SPC causes internalization of OGR1 in a structurally specific manner.",

author = "Yan Xu and Kui Zhu and Guiying Hong and Weihua Wu and Baudhuin, {Linnea M.} and Xiao, {Yi Jin} and Damron, {Derek S.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Bryan Willams and Dianne Perez for their critical reading of this manuscript. This work is supported in part by American Cancer Society grant RPG-99-062-01-CNE, an American Cancer Society OHIO Division grant, US Army grant DAMD 17-99-1-9563 to Y.X. and by Betsey De Windt endowment funds for the Department of Cancer Biology. Correspondence and requests for materials should be addressed to Y.X.",

year = "2000",

doi = "10.1038/35010529",

language = "English (US)",

volume = "2",

pages = "261--267",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "Nature Publishing Group",

number = "5",

}

TY - JOUR

T1 - Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1

AU - Xu, Yan

AU - Zhu, Kui

AU - Hong, Guiying

AU - Wu, Weihua

AU - Baudhuin, Linnea M.

AU - Xiao, Yi Jin

AU - Damron, Derek S.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Bryan Willams and Dianne Perez for their critical reading of this manuscript. This work is supported in part by American Cancer Society grant RPG-99-062-01-CNE, an American Cancer Society OHIO Division grant, US Army grant DAMD 17-99-1-9563 to Y.X. and by Betsey De Windt endowment funds for the Department of Cancer Biology. Correspondence and requests for materials should be addressed to Y.X.

PY - 2000

Y1 - 2000

N2 - Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to be mediated by the activation of unidentified G-protein-coupled receptors. Here we sinew that SPC is a high-affinity ligand for an orphan receptor, ovarian cancer G-protein-coupled receptor 1 (OGR1). In OGR1-transfected cells, SPC binds to OGR1 with high affinity (K(d) = 33.3 nM) and high specificity and transiently increases intracellular calcium. The specific binding of SPC to OGR1 also activates p42/44 mitogen-activated protein kinases (MAP kinases) and inhibits cell proliferation. In addition, SPC causes internalization of OGR1 in a structurally specific manner.

AB - Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to be mediated by the activation of unidentified G-protein-coupled receptors. Here we sinew that SPC is a high-affinity ligand for an orphan receptor, ovarian cancer G-protein-coupled receptor 1 (OGR1). In OGR1-transfected cells, SPC binds to OGR1 with high affinity (K(d) = 33.3 nM) and high specificity and transiently increases intracellular calcium. The specific binding of SPC to OGR1 also activates p42/44 mitogen-activated protein kinases (MAP kinases) and inhibits cell proliferation. In addition, SPC causes internalization of OGR1 in a structurally specific manner.

UR - http://www.scopus.com/inward/record.url?scp=0033772419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033772419&partnerID=8YFLogxK

U2 - 10.1038/35010529

DO - 10.1038/35010529

M3 - Article

C2 - 10806476

AN - SCOPUS:0033772419

SN - 1465-7392

VL - 2

SP - 261

EP - 267

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 5

ER -

Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this