Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer

Miralem Mrkonjic, Nicole M. Roslin, Celia M. Greenwood, Stavroula Raptis, Aaron Pollett, Peter W. Laird, Vaijayanti V. Pethe, Theodore Chiang, Darshana Daftary, Elizabeth Dicks, Stephen N Thibodeau, Steven Gallinger, Patrick S. Parfrey, H. Banfield Younghusband, John D. Potter, Thomas J. Hudson, John R. McLaughlin, Roger C. Green, Brent W. Zanke, Polly A. NewcombAndrew D. Paterson, Bharati Bapat

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: We previously identified an association between a mismatch repair gene, MLH1, promoter SNP (rs1800734) and microsatellite unstable (MSI-H) colorectal cancers (CRCs) in two samples. The current study expanded on this finding as we explored the genetic basis of DNA methylation in this region of chromosome 3. We hypothesized that specific polymorphisms in the MLH1 gene region predispose it to DNA methylation, resulting in the loss of MLH1 gene expression, mismatch-repair function, and consequently to genome-wide microsatellite instability. Methodology/Principal Findings: We first tested our hypothesis in one sample from Ontario (901 cases, 1,097 controls) and replicated major findings in two additional samples from Newfoundland and Labrador (479 cases, 336 controls) and from Seattle (591 cases, 629 controls). Logistic regression was used to test for association between SNPs in the region of MLH1 and CRC, MSI-H CRC, MLH1 gene expression in CRC, and DNA methylation in CRC. The association between rs1800734 and MSI-H CRCs, previously reported in Ontario and Newfoundland, was replicated in the Seattle sample. Two additional SNPs, in strong linkage disequilibrium with rs1800734, showed strong associations with MLH1 promoter methylation, loss of MLH1 protein, and MSI-H CRC in all three samples. The logistic regression model of MSI-H CRC that included MLH1-promotermethylation status and MLH1 immunohisotchemistry status fit most parsimoniously in all three samples combined. When rs1800734 was added to this model, its effect was not statistically significant (P-value = 0.72 vs. 2.3×10-4 when the SNP was examined alone). Conclusions/Significance: The observed association of rs1800734 with MSI-H CRC occurs through its effect on the MLH1 promoter methylation, MLH1 IHC deficiency, or both.

Original languageEnglish (US)
Article numbere13314
JournalPLoS One
Volume5
Issue number10
DOIs
StatePublished - Oct 13 2010

Fingerprint

DNA methylation
DNA Methylation
colorectal neoplasms
Colorectal Neoplasms
protein synthesis
Genes
Association reactions
Proteins
Methylation
Single Nucleotide Polymorphism
genes
Gene expression
Microsatellite Repeats
Newfoundland and Labrador
Logistics
DNA Mismatch Repair
Repair
Logistic Models
promoter regions
Ontario

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mrkonjic, M., Roslin, N. M., Greenwood, C. M., Raptis, S., Pollett, A., Laird, P. W., ... Bapat, B. (2010). Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer. PLoS One, 5(10), [e13314]. https://doi.org/10.1371/journal.pone.0013314

Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer. / Mrkonjic, Miralem; Roslin, Nicole M.; Greenwood, Celia M.; Raptis, Stavroula; Pollett, Aaron; Laird, Peter W.; Pethe, Vaijayanti V.; Chiang, Theodore; Daftary, Darshana; Dicks, Elizabeth; Thibodeau, Stephen N; Gallinger, Steven; Parfrey, Patrick S.; Younghusband, H. Banfield; Potter, John D.; Hudson, Thomas J.; McLaughlin, John R.; Green, Roger C.; Zanke, Brent W.; Newcomb, Polly A.; Paterson, Andrew D.; Bapat, Bharati.

In: PLoS One, Vol. 5, No. 10, e13314, 13.10.2010.

Research output: Contribution to journalArticle

Mrkonjic, M, Roslin, NM, Greenwood, CM, Raptis, S, Pollett, A, Laird, PW, Pethe, VV, Chiang, T, Daftary, D, Dicks, E, Thibodeau, SN, Gallinger, S, Parfrey, PS, Younghusband, HB, Potter, JD, Hudson, TJ, McLaughlin, JR, Green, RC, Zanke, BW, Newcomb, PA, Paterson, AD & Bapat, B 2010, 'Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer', PLoS One, vol. 5, no. 10, e13314. https://doi.org/10.1371/journal.pone.0013314
Mrkonjic, Miralem ; Roslin, Nicole M. ; Greenwood, Celia M. ; Raptis, Stavroula ; Pollett, Aaron ; Laird, Peter W. ; Pethe, Vaijayanti V. ; Chiang, Theodore ; Daftary, Darshana ; Dicks, Elizabeth ; Thibodeau, Stephen N ; Gallinger, Steven ; Parfrey, Patrick S. ; Younghusband, H. Banfield ; Potter, John D. ; Hudson, Thomas J. ; McLaughlin, John R. ; Green, Roger C. ; Zanke, Brent W. ; Newcomb, Polly A. ; Paterson, Andrew D. ; Bapat, Bharati. / Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer. In: PLoS One. 2010 ; Vol. 5, No. 10.
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T1 - Specific variants in the MLH1 gene region may drive DNA methylation, loss of protein expression, and MSI-H colorectal cancer

AU - Mrkonjic, Miralem

AU - Roslin, Nicole M.

AU - Greenwood, Celia M.

AU - Raptis, Stavroula

AU - Pollett, Aaron

AU - Laird, Peter W.

AU - Pethe, Vaijayanti V.

AU - Chiang, Theodore

AU - Daftary, Darshana

AU - Dicks, Elizabeth

AU - Thibodeau, Stephen N

AU - Gallinger, Steven

AU - Parfrey, Patrick S.

AU - Younghusband, H. Banfield

AU - Potter, John D.

AU - Hudson, Thomas J.

AU - McLaughlin, John R.

AU - Green, Roger C.

AU - Zanke, Brent W.

AU - Newcomb, Polly A.

AU - Paterson, Andrew D.

AU - Bapat, Bharati

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N2 - Background: We previously identified an association between a mismatch repair gene, MLH1, promoter SNP (rs1800734) and microsatellite unstable (MSI-H) colorectal cancers (CRCs) in two samples. The current study expanded on this finding as we explored the genetic basis of DNA methylation in this region of chromosome 3. We hypothesized that specific polymorphisms in the MLH1 gene region predispose it to DNA methylation, resulting in the loss of MLH1 gene expression, mismatch-repair function, and consequently to genome-wide microsatellite instability. Methodology/Principal Findings: We first tested our hypothesis in one sample from Ontario (901 cases, 1,097 controls) and replicated major findings in two additional samples from Newfoundland and Labrador (479 cases, 336 controls) and from Seattle (591 cases, 629 controls). Logistic regression was used to test for association between SNPs in the region of MLH1 and CRC, MSI-H CRC, MLH1 gene expression in CRC, and DNA methylation in CRC. The association between rs1800734 and MSI-H CRCs, previously reported in Ontario and Newfoundland, was replicated in the Seattle sample. Two additional SNPs, in strong linkage disequilibrium with rs1800734, showed strong associations with MLH1 promoter methylation, loss of MLH1 protein, and MSI-H CRC in all three samples. The logistic regression model of MSI-H CRC that included MLH1-promotermethylation status and MLH1 immunohisotchemistry status fit most parsimoniously in all three samples combined. When rs1800734 was added to this model, its effect was not statistically significant (P-value = 0.72 vs. 2.3×10-4 when the SNP was examined alone). Conclusions/Significance: The observed association of rs1800734 with MSI-H CRC occurs through its effect on the MLH1 promoter methylation, MLH1 IHC deficiency, or both.

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