Specific modulation of cyclic ADP-ribose-induced Ca2+ release by polyamines

Eduardo Nunes Chini, K. W. Beers, C. C S Chini, T. P. Dousa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Cyclic ADP-ribose (cADPR) is a potent mediator of Ca2+ mobilization from intracellular stores in sea urchin eggs. However, the regulation of the cADPR-induced Ca2+ release system is not yet fully elucidated. We now report that spermine and related polyamines, in physiological concentrations, were able to inhibit the Ca2+ release induced by cADPR in sea urchin egg homogenate bioassays, as measured using the Ca2+ indicator fluo 3, but had no effect on the Ca2+ release induced by D-myo-inositol 1,4,5- trisphosphate (IP3) or by nicotinate adenine dinucleotide phosphate (NAADP). Spermine was a more potent inhibitor of the cADPR-induced Ca2+ release than spermidine and putrescine. Spermine inhibited not only the release induced by cADPR but also the Ca2+ release induced by caffeine and ryanodine. Finally, pretreatment of the sea urchin egg homogenates with caffeine or Sr2+ and Ca2+ prevented the inhibitory effect of spermine on cADPR-induced Ca2+ release. We propose that polyamines, which are present in millimolar concentrations in fertilized eggs, are specific inhibitors of the ryanodine channel and perhaps may serve as endogenous regulators of the cADPR-induced Ca2+ release system.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume269
Issue number4 38-4
StatePublished - 1995

Fingerprint

Cyclic ADP-Ribose
ribose
Polyamines
polyamines
Modulation
Spermine
calcium
Sea Urchins
spermine
Ryanodine
Echinoidea
Caffeine
Ovum
caffeine
Inositol 1,4,5-Trisphosphate
Putrescine
Spermidine
Bioassay
Zygote
Biological Assay

Keywords

  • caffeine
  • calcium
  • inositol 1,4,5- trisphosphate
  • intracellular calcium
  • nicotinate adenine dinucleotide phosphate
  • putrescine
  • ryanodine
  • sea urchin egg
  • spermidine
  • spermine

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Specific modulation of cyclic ADP-ribose-induced Ca2+ release by polyamines. / Chini, Eduardo Nunes; Beers, K. W.; Chini, C. C S; Dousa, T. P.

In: American Journal of Physiology - Cell Physiology, Vol. 269, No. 4 38-4, 1995.

Research output: Contribution to journalArticle

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