TY - JOUR
T1 - Specific inhibition of myelination by lead in vitro; Comparison with arsenic, thallium, and mercury
AU - Windebank, Anthony J.
N1 - Funding Information:
Abbreviations: DRG-dorsal root ganglion, AN&standard flameless atomic absorption, HPS-human placental serum. ’ The expert technical assistance of Marceil D. Blexrud and secretarial assistance of Linda A. Goldbeck is appreciated. Estimations of metal concentrations were carried out in the Mayo Metals Laboratory by Dr. Thomas P. Moyer. This work was supported by National Institutes of Health grants NS 00681 and NS 14304 PlO and was presented in part at the 38th Annual Meeting of the American Academy of Neurology in New Orleans, LA, in April of 1986.
PY - 1986/10
Y1 - 1986/10
N2 - Lead induces peripheral nerve segmental demyelination in rats. Arsenic and thallium produce a peripheral neuropathy characterized by axonal degeneration in humans. Mercury and thallium appear to damage both the peripheral and the central nervous system. It is not known whether this difference in effect is due to different molecular forms of the elements, to differential access to various compartments of the nervous system, or to intrinsically different properties of the elements. Using an in vitro model system of dorsal root ganglion neurons and morphometry of neurite outgrowth and myelination, we demonstrated that mercury and arsenic produce 50% inhibition of neurite outgrowth at 3.9 and 9.6 × 10-6 M, respectively, whereas the same degree of inhibition is produced by 1.3 × 10-4 M thallium and 3.3 × 10-4 M lead. Lead also produces complete inhibition of myelination at 1 × 10-6 M, suggesting that a primary effect on myelination is present in this model system as well as in the intact rodent.
AB - Lead induces peripheral nerve segmental demyelination in rats. Arsenic and thallium produce a peripheral neuropathy characterized by axonal degeneration in humans. Mercury and thallium appear to damage both the peripheral and the central nervous system. It is not known whether this difference in effect is due to different molecular forms of the elements, to differential access to various compartments of the nervous system, or to intrinsically different properties of the elements. Using an in vitro model system of dorsal root ganglion neurons and morphometry of neurite outgrowth and myelination, we demonstrated that mercury and arsenic produce 50% inhibition of neurite outgrowth at 3.9 and 9.6 × 10-6 M, respectively, whereas the same degree of inhibition is produced by 1.3 × 10-4 M thallium and 3.3 × 10-4 M lead. Lead also produces complete inhibition of myelination at 1 × 10-6 M, suggesting that a primary effect on myelination is present in this model system as well as in the intact rodent.
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U2 - 10.1016/0014-4886(86)90283-9
DO - 10.1016/0014-4886(86)90283-9
M3 - Article
C2 - 3758280
AN - SCOPUS:0022535099
SN - 0014-4886
VL - 94
SP - 203
EP - 212
JO - Neurodegeneration
JF - Neurodegeneration
IS - 1
ER -