Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors

Zachary W. Ulissi; Fatih Sen; Xun Gong; Selda Sen; Nicole Iverson; Ardemis A. Boghossian; Luiz C. Godoy; Gerald N. Wogan; Debabrata Mukhopadhyay; Michael S. Strano

doi:10.1021/nl502338y

Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors

Zachary W. Ulissi, Fatih Sen, Xun Gong, Selda Sen, Nicole Iverson, Ardemis A. Boghossian, Luiz C. Godoy, Gerald N. Wogan, Debabrata Mukhopadhyay, Michael S. Strano

Jacksonville, FL

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.

Original language	English (US)
Pages (from-to)	4887-4894
Number of pages	8
Journal	Nano Letters
Volume	14
Issue number	8
DOIs	https://doi.org/10.1021/nl502338y
State	Published - Aug 13 2014

Keywords

Single-walled carbon nanotube
nitric oxide
photoluminescence
sensor

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl502338y

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title = "Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors",

abstract = "Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.",

keywords = "Single-walled carbon nanotube, nitric oxide, photoluminescence, sensor",

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T1 - Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors

AU - Ulissi, Zachary W.

AU - Sen, Fatih

AU - Gong, Xun

AU - Sen, Selda

AU - Iverson, Nicole

AU - Boghossian, Ardemis A.

AU - Godoy, Luiz C.

AU - Wogan, Gerald N.

AU - Mukhopadhyay, Debabrata

AU - Strano, Michael S.

PY - 2014/8/13

Y1 - 2014/8/13

N2 - Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.

AB - Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.

KW - Single-walled carbon nanotube

KW - nitric oxide

KW - photoluminescence

KW - sensor

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Spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors

Abstract

Keywords

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