@article{ccff5e5f1f344b05ba9db3ff1ffe40fc,
title = "Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease",
abstract = "Heterogeneity of lymphatic vessels during embryogenesis is critical for organ-specific lymphatic function. Little is known about lymphatics in the developing kidney, despite their established roles in pathology of the mature organ. We performed three-dimensional imaging to characterize lymphatic vessel formation in the mammalian embryonic kidney at single-cell resolution. In mouse, we visually and quantitatively assessed the development of kidney lymphatic vessels, remodeling from a ring-like anastomosis under the nascent renal pelvis, a site of VEGF-C expression, to form a patent vascular plexus. We identified a heterogenous population of lymphatic endothelial cell clusters in mouse and human embryonic kidneys. Exogenous VEGF-C expanded the lymphatic population in explanted mouse embryonic kidneys. Finally, we characterized complex kidney lymphatic abnormalities in a genetic mouse model of polycystic kidney disease. Our study provides novel insights into the development of kidney lymphatic vasculature; a system which likely has fundamental roles in renal development, physiology and disease.",
author = "Jafree, {Daniyal J.} and Dale Moulding and Maria Kolatsi-Joannou and Tejedor, {Nuria Perretta} and Price, {Karen L.} and Natalie Milmoe and Claire Walsh and Correra, {Rosa Maria} and Winyard, {Paul J.D.} and Harris, {Peter C.} and Christiana Ruhrberg and Simon Walker-Samuel and Riley, {Paul R.} and Woolf, {Adrian S.} and Scambler, {Peter J.} and Long, {David A.}",
note = "Funding Information: We thank Professor Juan Pedro Martinez-Barbera (UCL), Dr Peter Baluk (University of California San Francisco) and Dr Nicolas Renier (Institut du Cerveau et de la Moelle Epiniere) for help and advice. All mice were maintained by staff at GOSICH Western Laboratories and UCL Biological Services. Microscopy was performed at the Light Microscopy Core Facility at UCL GOSICH. All work was performed with the support of the National Institute for Health Research (NIHR) Biomedical Research Centre at Great Ormond Street Hospital for Children NHS Foundation Trust and University College London. Funding This work was funded by a Child Health Research Studentship from UCL Great Ormond Street Institute of Child Health (DJJ, PJS, DAL), the UCL MB/PhD Programme (DJJ), the Medical Research Council (MR/P018629/1 to DAL and ASW, MR/L002744/1 to ASW, MR/K026739/1 to ASW), Diabetes UK (15/0005283 to DAL), Kidney Research UK (Paed_RP_10_2018 to DAL, ASW and DJJ, IN_012_2019 to DAL and DJJ), The British Heart Foundation (CH/11/1/28798 to PRR, RG/15/14/31880 to PJS and FS/19/14/34170 to RMC) and a NIHR Great Ormond Street Hospital Biomedical Research Centre Award (17DD08, (DM)). Funding Information: This work was funded by a Child Health Research Studentship from UCL Great Ormond Street Funding Information: UCL GOSICH. All work was performed with the support of the National Institute for Health Publisher Copyright: {\textcopyright} 2019, eLife Sciences Publications Ltd. All rights reserved.",
year = "2019",
month = dec,
doi = "10.7554/eLife.48183",
language = "English (US)",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}