Spaceflight results in reduced mRNA levels for tissue-specific proteins in the musculoskeletal system

P. Backup, K. Westerlind, S. Harris, T. Spelsberg, B. Kline, R. Turner

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The purpose of the present study in growing rats was to investigate the effects of short-term spaceflight on gene expression in bone and muscle and on cortical bone histomorphometry. Two experiments were carried out; Physiological Systems Experiments 1 and 2 were 4- and 10-day flights, respectively. Radial bone growth in the humerus was unchanged during the 4- day flight and decreased during the 10-day flight. Expression of mRNA for glyceraldehyde-3-phosphate dehydrogenase was unchanged in biceps, calvarial periosteum, and long-bone periosteum after spaceflight. Similarly, no changes in ribosomal RNA levels were observed in long-bone or calvarial periosteum after spaceflight. In contrast, spaceflight decreased steady-state mRNA levels for actin in muscle (4-day flight). Osteocalcin (both spaceflights) and the prepro-α2[I] chain of type I precollagen (10-day flight) mRNA levels were decreased in long-bone and calvarial periosteum after spaceflight. These results indicate that the effects of spaceflight on the musculoskeletal system include decreased expression of some muscle- and bone-specific genes as well as decreased bone formation. Interestingly, detectable reductions in gene expression for bone matrix proteins preceded histological evidence for decreased bone formation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume266
Issue number4 29-4
StatePublished - 1994

Fingerprint

Musculoskeletal system
Space Flight
Musculoskeletal System
Bone
Periosteum
Tissue
Messenger RNA
Bone and Bones
Proteins
Osteogenesis
Muscles
Muscle
Gene expression
Gene Expression
Glyceraldehyde-3-Phosphate Dehydrogenases
Bone Matrix
Ribosomal RNA
Bone Development
Osteocalcin
Humerus

Keywords

  • bone metabolism
  • calvariae
  • disuse osteopenia
  • gene expression
  • long bones
  • matrix proteins
  • Northern blot analysis
  • rat bone
  • weight bearing

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Spaceflight results in reduced mRNA levels for tissue-specific proteins in the musculoskeletal system. / Backup, P.; Westerlind, K.; Harris, S.; Spelsberg, T.; Kline, B.; Turner, R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 266, No. 4 29-4, 1994.

Research output: Contribution to journalArticle

Backup, P. ; Westerlind, K. ; Harris, S. ; Spelsberg, T. ; Kline, B. ; Turner, R. / Spaceflight results in reduced mRNA levels for tissue-specific proteins in the musculoskeletal system. In: American Journal of Physiology - Endocrinology and Metabolism. 1994 ; Vol. 266, No. 4 29-4.
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