Sources of blood glycerol during fasting

Michael Dennis Jensen, Visvanathan Chandramouli, William C. Schumann, Karin Ekberg, Stephen F. Previs, Sameer Gupta, Bernard R. Landau

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To determine the source(s) of blood and very low density lipoprotein (VLDL)-triglyceride glycerol during fasting, four men ingested 2H2O from 14 to 20 h into a 60-h fast to achieve ∼0.5% body water enrichment. At 60 h of fasting, glycerol flux was measured using [2-14C]glycerol. Blood was taken for measurement of 2H enrichment at carbon 6 of glucose and at carbon 3 of free glycerol and VLDL-triglyceride glycerol. 2H enrichment of the 2 hydrogens bound to carbon 3 of VLDL-triglyceride glycerol was 105 ± 2% of the 2H enrichment of the 2 hydrogens bound to carbon 6 of glucose, indicating isotopic equilibrium between hepatic glyceraldehyde 3-P and glycerol 3-P. The 2H enrichment of the 2 hydrogens bound to carbon 3 of free glycerol was 17 ± 3% of VLDL-triglyceride glycerol, indicating that a significant percentage of free glycerol in blood originated from the hydrolysis of circulating VLDL-triglyceride or a pool of glycerol with similar 2H enrichment. Glycerol flux was 6.3 ± 1.1 μmol·kg-1·min-1. Glycerol appearing from nonadipose tissue sources was then ∼1.1 μmol·kg-1·min-1. Seven other subjects were fasted for 12, 42, and 60 h. A small percentage of glycerol in the circulation after 12 h of fasting was enriched with 2H. The enrichment of the 2 hydrogens bound to carbon 3 of free glycerol in the longer periods of fasting was ∼16% of the enrichment of the 2 hydrogens bound to carbon 6 of glucose. Therefore, as much as 15-20% of systemic glycerol turnover during fasting is not from lipolysis of adipose tissue triglyceride.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume281
Issue number5 44-5
StatePublished - 2001

Fingerprint

Glycerol
Fasting
Blood
Carbon
Hydrogen
Glucose
Tissue
Fluxes
Glyceraldehyde
Body Water
Lipolysis
Adipose Tissue
Hydrolysis
Triglycerides

Keywords

  • Deuterated water
  • Lipolysis
  • Triglyceride
  • Very low density lipoprotein

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Jensen, M. D., Chandramouli, V., Schumann, W. C., Ekberg, K., Previs, S. F., Gupta, S., & Landau, B. R. (2001). Sources of blood glycerol during fasting. American Journal of Physiology - Endocrinology and Metabolism, 281(5 44-5).

Sources of blood glycerol during fasting. / Jensen, Michael Dennis; Chandramouli, Visvanathan; Schumann, William C.; Ekberg, Karin; Previs, Stephen F.; Gupta, Sameer; Landau, Bernard R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 281, No. 5 44-5, 2001.

Research output: Contribution to journalArticle

Jensen, MD, Chandramouli, V, Schumann, WC, Ekberg, K, Previs, SF, Gupta, S & Landau, BR 2001, 'Sources of blood glycerol during fasting', American Journal of Physiology - Endocrinology and Metabolism, vol. 281, no. 5 44-5.
Jensen MD, Chandramouli V, Schumann WC, Ekberg K, Previs SF, Gupta S et al. Sources of blood glycerol during fasting. American Journal of Physiology - Endocrinology and Metabolism. 2001;281(5 44-5).
Jensen, Michael Dennis ; Chandramouli, Visvanathan ; Schumann, William C. ; Ekberg, Karin ; Previs, Stephen F. ; Gupta, Sameer ; Landau, Bernard R. / Sources of blood glycerol during fasting. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 281, No. 5 44-5.
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AB - To determine the source(s) of blood and very low density lipoprotein (VLDL)-triglyceride glycerol during fasting, four men ingested 2H2O from 14 to 20 h into a 60-h fast to achieve ∼0.5% body water enrichment. At 60 h of fasting, glycerol flux was measured using [2-14C]glycerol. Blood was taken for measurement of 2H enrichment at carbon 6 of glucose and at carbon 3 of free glycerol and VLDL-triglyceride glycerol. 2H enrichment of the 2 hydrogens bound to carbon 3 of VLDL-triglyceride glycerol was 105 ± 2% of the 2H enrichment of the 2 hydrogens bound to carbon 6 of glucose, indicating isotopic equilibrium between hepatic glyceraldehyde 3-P and glycerol 3-P. The 2H enrichment of the 2 hydrogens bound to carbon 3 of free glycerol was 17 ± 3% of VLDL-triglyceride glycerol, indicating that a significant percentage of free glycerol in blood originated from the hydrolysis of circulating VLDL-triglyceride or a pool of glycerol with similar 2H enrichment. Glycerol flux was 6.3 ± 1.1 μmol·kg-1·min-1. Glycerol appearing from nonadipose tissue sources was then ∼1.1 μmol·kg-1·min-1. Seven other subjects were fasted for 12, 42, and 60 h. A small percentage of glycerol in the circulation after 12 h of fasting was enriched with 2H. The enrichment of the 2 hydrogens bound to carbon 3 of free glycerol in the longer periods of fasting was ∼16% of the enrichment of the 2 hydrogens bound to carbon 6 of glucose. Therefore, as much as 15-20% of systemic glycerol turnover during fasting is not from lipolysis of adipose tissue triglyceride.

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