Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability

Hui Yuan Tseng, Niko Thorausch, Tilman Ziegler, Alexander Meves, Reinhard Fässler, Ralph T. Böttcher

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Trafficking of α5β1 integrin to lysosomes and its subsequent degradation is influenced by ligand occupancy and the binding of SNX17 via its protein 4.1, ezrin, radixin, moesin (FERM) domain to the membrane-distal NPxY motif in the cytoplasmic domain of β1 integrin in early endosomes. Two other sorting nexin (SNX) family members, namely SNX27 and SNX31, share with SNX17 next to their obligate phox domain a FERM domain, which may enable them to bind β integrin tails. Here we report that, in addition to SNX17, SNX31 but not SNX27 binds several β integrin tails in early endosomes in a PI3 (phosphatidylinositide 3)-kinase-dependent manner. Similarly like SNX17, binding of SNX31 with β1 integrin tails in early endosomes occurs between the FERM domain and the membrane-distal NPxY motif in the β1 integrin cytoplasmic domain. Furthermore, expression of SNX31 rescues β1 integrin surface levels and stability in SNX17-depleted cells. In contrast to SNX17, expression of SNX31 is restricted and found highly expressed in bladder and melanoma tissue. Altogether, these results demonstrate that SNX31 is an endosomal regulator of β integrins with a restricted expression pattern.

Original languageEnglish (US)
Pages (from-to)3180-3194
Number of pages15
JournalJournal of Molecular Biology
Volume426
Issue number18
DOIs
StatePublished - Sep 9 2014

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Sorting Nexins
Integrins
Endosomes
Membranes
Lysosomes
Melanoma
Urinary Bladder
Phosphotransferases
Ligands

Keywords

  • integrin trafficking
  • melanoma
  • protein turnover
  • SNX17
  • SNX27

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability. / Tseng, Hui Yuan; Thorausch, Niko; Ziegler, Tilman; Meves, Alexander; Fässler, Reinhard; Böttcher, Ralph T.

In: Journal of Molecular Biology, Vol. 426, No. 18, 09.09.2014, p. 3180-3194.

Research output: Contribution to journalArticle

Tseng, Hui Yuan ; Thorausch, Niko ; Ziegler, Tilman ; Meves, Alexander ; Fässler, Reinhard ; Böttcher, Ralph T. / Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability. In: Journal of Molecular Biology. 2014 ; Vol. 426, No. 18. pp. 3180-3194.
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